应用磁激活细胞分选法从母血中分离胎儿有核红细胞做FISH行无创性产前诊断的研究.pdfVIP

F ISH 19 2697 F ISH ! 任景慧 赵跃宏 徐宏里 林琳华 518020 刘 俞 李富荣 王新根 宋兰林 R714 A ( 2006) ∀ # : , : 14 18~ 32 20m l, CD14CD 45CD7 1 K le ihaur- Be ke ( K - B) ( F ISH ) : ( nuc lea ed red b lood ce ll, NR BC) , K - B F ISH F ISH , , 100% , 9286% ; 9286% , 100% : , F ISH ∀# M ACS F ISH N on- invasive Prenatal D iagnosis From M aternal B lood: F SH of Fetal Nucleated Erythrocytes solated by M agnetic A ctivated Cell Sorting REN Jing - Hu,i ZHAO Yue-H ong, XUH ong - L i, et alD epartmentof Obstetrics and ynecology, thePeop lesH osp ital of Shenzhen, the SecondAff iliatedH osp ital of theM edical College of Jinan University, Shenzhen 518020, China ∃Abstract% Objective: Fe al nuc lea ed red b lood cells ( NR BCs) are ra re in m a ernal c ircula ion, bu he re presence cons i u es a po en ia l source o f non- invasive prena a l g ene ic d iagnosis. Th is s udy w as under aken o es ablish a non- invasive prena a l g ene ic d iagno sis m e hod u sing isola ed fe al NRBCs. M ethods: T he s udy popula ion inc luded 14 pa ien s from 20 o 32 w eeks o f ges a ion. A mu l i- s ep m e hod including sing le densi y grad ien , nega ive and pos i ive m agne ic ac iva ed ce ll sor ing (M ACS) u sing CD 14, CD 45 and CD 71, K le ihaur- Be ke s a in ing, and fluo rescence in si u hybr id iza ion ( F ISH ) w as performed. Resu lts: F e a lNRBC s w ere sepa ra ed from a ll ca ses and conf irm ed by K - B s ain and F ISH . T he fe a l sex de erm ina ion con firm ed by cordocen es is show ed 100% sensi iv i y and 9286% spec