乙醇发酵菌株耐毒特性初步研究.pdfVIP

中国可再生能源学会2011 年学术年会论文（生物质能） 乙醇发酵菌株耐毒特性的初步研究 曹萌 门珣 杨非 郝学才 翟冬梅 杨秀山 田沈* (首都师范大学生命科学学院，北京 摘要：本文为了研究实验室乙醇发酵菌株的耐毒特性，评估了不同浓度的糠醛对融合菌株Y10-F 生长的影响，并以实验室其他两株高效乙醇发酵菌株Y1、Y5为对照，利用未经任何脱毒处理的 蒸汽爆破并酶解的玉米秸秆为发酵底物进行乙醇发酵。当细胞浓度为1.5g/L时，仅Y1能在72h 将葡萄糖消耗完，当细胞浓度为3.0g/L时，三株菌均能在60h内将水解液中的所有的葡萄糖消 耗完，并都具有较高的乙醇产量，体现了三株菌较好的耐毒和产乙醇能力。 关键词：酵母菌株；糠醛；蒸汽爆破；玉米秸秆；乙醇 Studyonthetoxicresistanceofyeaststrainsforethanolproduction Studyonthetoxicresistanceofyeaststrainsforethanolproduction SSttuuddyyoonntthheettooxxiiccrreessiissttaanncceeooffyyeeaassttssttrraaiinnssffoorreetthhaannoollpprroodduuccttiioonn CaoMeng MenXun YangFei HaoXuecai YangXiushan TianShen CaoMeng MenXun YangFei HaoXuecai YangXiushan TianShen CCaaooMMeenngg，MMeennXXuunn，YYaannggFFeeii，HHaaooXXuueeccaaii，YYaannggXXiiuusshhaann，TTiiaannSShheenn (College oflifeScience,Captial Normal University,Beijing,China) Abstract: Abstract: AAbbssttrraacctt:: This research evaluated the inhibitory effect of various concentrations of furfural on growth of the Y10-F for study on the abilities of toxic tolerance. Using the two yeast strains of Y5 and Y1 developed in our lab strain, as controls. Using the non-detoxified enzymatic hydrolysate of corn stover pretreated by steam-explosion as the fermentation substrate. When the cell concentration is 1.5g/L, onlyY1 couldconsumedall the glucosewithin 72hours.Whenthe cellconcentration is 3.0g/L, allY1、 Y5 and Y10-F could consumed all the glucose within 60 hours with high ethanol yield. All of three strainsexpressedsufficientabilitiesoftoxic-tolerantandethanolproduction. Keywords: Keywords: KKeeyywwoorrddss::Yeaststrain;furfural;Steam-explosion; Cornstover; ethanol [2] 1 引言