黄连须和小檗碱对草鱼非特异性免疫系统的影响及对嗜水气单胞菌感染的抵抗作用（英文）.pdfVIP

第39卷 第2期 水 生 生 物 学 报 Vo1．39，N o．2 2015年 3月 ACTA HYDR0B10L0GICA SINICA M ar．． 2015 doi：10．7541／2015．35 EFFECToFFIBRoUSRooToFC’DP刀 C!日=，=、『 、『 FRANCH AND BERBERINE oN THENoN．SPECIFIC IMM UNITYAND RESiSTANCEAGAINST 豫 D 月 D DP日 INFECTIoNINGRASSCARP(CTENOPHARYNGODONIDELLA) MOU Shao—Xia，ZHOU Xia，PENGYao—ZongandLIXue—Gang (SouthwestUniversity，SchoolofPharmaceuticalSciences，Chongqing400715，China) Abstract：TodeterminetheeffectsofFibrousrootofCoptischinensisFranch(FRC)andberberine(BBR)on non．specificimmunityanddiseaseresistanceagainstAeromonashydrophilainfectioningrasscarp (Cteno— pharyngodonidella)，fishwerefedwithdietscontaining1．00％，0．50％，0．25％，0．10％，0．O0％ofFRCpowderand 0．05％ ofBBR for28days．FishwerechallengedwithA．hydrophila28dayspostfeedingandsurvivalrate(．R) wasrecordedover14dayspostchallenge．Nitrobluetetrazolium (NBT)activity,serllmlysozymeactivity，phago- cyticactivityandcomplementC3levelwerestudiedtoassesstheimmuneresponseOffish．Theresultsrevealed thatdietaryFRC andBBR increasetheNBT activity，serum lysozymeactivity,phagocyticactivityandcomple— mentC3levelingrasscarlawhencomparedtothemodelgroupfMD1．Feedcontaining0．50％FRCwasthemost effectivewiththeJSR ofthefishsignificantlyincreasedbv44％ comparedtoMD．Theinvitroantibacterialactivity 0fFRCandBBRwereinvestigatedwiththeminimuminhibitoryconcentration(MIC)methodandshowedstrong inhibitory effects．Theresultsindicated thatFRC andBBR canbeusedasimmunostimulantsto enhancethe non—specificimmunityanddiseaseresistanceofgrasscapr againstA．hydrophila． Keywords：FibrousrootofCoptischinensisFranch(FRC)；Berberine(BBR)；Grasscarp；Non—specificimmunity； Aeromonashydrophila；Antibacterialactivity；Survivalrate CLCnumber：$968