食用菌中转基因成分的多重PCR检测技术的研究.pdfVIP

TH Symposium of the 5 International Conference on Transgenic Animals 步了解了转染细胞在EBV TPA 协同处理后的 由于EBV 和TPA 之间可能存在着相互颉抗的 基因表达变化 这些基因的改变可能会与鼻咽 作用 致使基因表达水平未发生改变或无明显 癌的发生 发展有关 为我们能进一步研究 变化 EBV 和TPA 之间的相互颉抗作用机制还 EBV TPA 对细胞影响的分子机理以及鼻咽癌 有待于进一步研究 的发病机理奠定基础 此外 某些表达水平未 (Figures, tables and references omitted) 改变的基因也可能参与了NPC 的发病过程 但 0604 Detection of geneticcally modified component in familiar edulis fungi by multiplex PCR 食用食用 食用食用 菌中转基因成分的多重菌中转基因成分的多重PCR检测技术研究检测技术研究 菌中转基因成分的多重菌中转基因成分的多重 检测技术研究检测技术研究 CHEN Wen-Bing1 陈文炳 JIANG Shu-Xun1 江树勋 SHAO Bi-Ying1 邵碧英 LI Shou-Song1 李寿崧 ZHU Xiao-Nan1 朱晓南 WANG Ze-Sheng2 王泽生 LIAO Jian-Hua2 廖剑华 1. Fujian Entry-Exit Inspection and Quarantine Bureau Fuzhou 350003 China; 2. Fujian Research Institute of Light Industry, Fuzhou 350005,China) Abstract DNA extracted from plasmid vector (p301-bG1) used in transgenic research of familiar edulis fungi was added into edulis fungy samples as simulated positive samples. The multiplex PCR was used to detect genetically modified component in 19 familiar edulis fungi in present study. The total DNAs were extracted from any samples by CTAB method. We developed multiplex PCR detection method for the target DNA fragments with the size of 165bp, 398bp, 545bp and 600bp of four transgenes, i.e. NOS, BAR, GUS and NPT-II respectively. Five combinations of duplex PCR including NOS-GUS, BAR-GUS, NOS-BAR, NOS-NPT II and BAR-NPT II, as well as 2 combinations of triplex PCR including NOS-BAR-GUS and NOS-BAR-NPT II, were amplified in present study. 食用菌中转基因成分的多重食用菌中转基因成分的多重PCR 检测技术研究检测技术研究 食用菌中转基因成分的多重食用菌中转基因成分的多重 检测技术研究检测技术研究 陈文炳 江树勋 邵碧英 李寿崧 朱晓南 王泽生 廖剑华 1 1 1 1 1 2 2 1 福建出入境检验检疫局 福州 3