内生真菌对福建金线莲栽培及酶活性的影响.pdfVIP

* 唐明娟, 孟志霞, 郭顺星 , 陈晓梅, 肖培根(, 100094) : 研究在野外盆栽条件下内生真菌AR18对福 金线莲(Anoectochilus roxburghii)生长发育的影响 福 金 线莲组培苗接菌移栽于草炭土或蛭石基质中, 1年后收获, 观察苗的生长势, 统计成活率, 称鲜重和干重, 同时测定1, 3葡聚 糖酶几 丁质酶多酚氧化酶和苯丙氨酸解氨酶活性 在草炭土和蛭石基质上, 接菌的福 金线莲苗成活率均达 100% ; 真菌对福 金线莲鲜重的增加有极显著促进作用 (P 001), 分别为对照组的 177和 183倍; 真菌对福 金线莲干重 的增加有显著促进作用 (P 005), 分别为对照组的 113和 128倍接菌后 4种酶活性均高于对照组 在福 金线 莲的家栽过程中可利用内生真菌提高成活率和产量 : 内生真菌; 福 金线莲; 移栽; 1, 3葡聚糖酶; 几 丁质酶; 多酚氧化酶; 苯丙氨酸解氨酶 : S567 : A : 1001- 2494( 2008) 12- 0890- 04 Effects of Endophytic Fungi on the Culture and Four Enzym e A ctivities ofA noectoch ilus roxburg h ii * TANG M i gjua , MENG Zh ixia, GUO Shu xi g , CHEN X iaome,i X IAO Pe ge (Institute of M edicinal Plants De velopment, ChineseAcademy of M edicalSciences and Peking nionM edical College, Beijing 100094, China) AB STRACT: OBJECT VE To study the effect of e dophytic fu gi AR18 (Epulorhiza sp ) o the grow th ofAnoectochilus rox burghiiMETHOD S Aroxburghiiwas i cubatedw ith e dophytic fu giAR18, harvested after bei g cultured for age i peat or ver m iculitem edium Seedli g s grow th vigorw ere observed, statisticsw ere take , fresh w eight a d dry weight w ere gai ed, a d e zym e activities of 1, 3gluca ase, chiti ase, polyphe oloxidase( PPO ) a d phe ylala i e amm o ialyase( PAL ) w ere determ i ed RE SULTS I peat a d verm icu lite m ed ium, the survival rates ofA roxburghii i cubated w ith e dophytic fu giw ere both 100% The fu gi extrem ely sig ifica tly i creased the fresh weight ofA roxburghii seed li gs(P 001), wh ich w as 177 tmi es a d 183 tmi es as that of the o i ocu lated o es, respectivelyThe fu gi sig ifica tly i creased the dry w eigh t of seed li gs(P 005), wh ich w as 113 tmi es a d 128 tmi es as co trol, respectivelyThe four e zym e activities were e ha ced i the seedli gs i cubated w ith e dophytic fu gi, compared w ith the co trolCONCLUS ON Survival rates a d yield ofA ro