补肾健脾药物血清对体外培养的成骨细胞增殖与分化的影响.pdfVIP

维普资讯 · 772· 中国中医药信息杂志 2004年 9月第 11卷第 9期 补肾健脾药物血清对体外培养的成骨细胞 增殖与分化的影响 任艳玲，郑洪新，杜 松 (辽宁中医学院，辽宁 沈阳 l10032) 摘要：目的 观察补肾健脾药物血清对新生大鼠颅骨成骨细胞增殖和分化的影响。方法 用多次酶消化法分 离培养新生大鼠颅盖骨成骨细胞，加入含不同药物浓度的补肾健脾药物血清条件培养液共同培养；改良Gomori钙 钴法染色鉴定，MTT法测定细胞增殖率，对硝基苯酚法检测碱性磷酸酶 (ALP)活性。骨疏康和尼尔雌醇作为阳性对 照药物 。结果 补肾健脾 中药组成骨细胞的增殖、ALP活性均显著高于空白对照组 (P0．05)，与骨疏康和尼尔雌 醇组比较有显著性差异。结论 补肾健脾 中药可通过增加成骨细胞的增殖与分化而促进骨形成。 关键词：成骨细胞；增殖；碱性磷酸酶；补肾健脾 中图分类号：R289．II 文献标识码：A 文章编号：1005—5304(2004)09—0772．03 冒 衄t_I 戮囊rdh of the Serum C．珊 TImI 嘲 锄d B．蝴 陆g s誓Ik嘶 日幽 F珊雠吐蛐 to Ⅱ P】m衄fa阳廿0na血d~ Olgl0fn V m—CⅡl忱m目Il0st伽 I嘲 RENYah． 1ing，ZHENGHong·xin，DUSong(LiaoningCollegeofTCM,Shenyang110032，ChinaJ Ah—眦 ：Ob~cflw Toobservetheeffectsoftheserum containedtonic—kidneyandbenefiting— spleen herbs to ht e prollferation na d differentiation of osteoblasts of newly born rats’ cranium ．劓 Il_1．dW e separated ht e osteoblastsofnewly born rats’crna ium and cultured ht em in vitro with ht e meht od of twice enzyme—digestion． And supplying the osteoblasts drug— contained serum which has different concentration． We identified the cells by mena s of Gomoristaining meht od na d detected ht e proliferation rate ofthe cells by MTT．The activity of htealkalinephosphataseofhtecellswasexaminedby htemethodofpraa—nitrylpheno1．An d we also chose ht e Gushukang na d nflestriol as ht e conrtoldur gs．Reenlb The Chinese herbs which have ht e function of tonifying the kidney na d benefiting ht e spleen cna stimulate ht e proliferation of ht e osteoblasts na d ht e activity of the alkaline phosphatase， which ra e dram atically better ht na the blna k—conrtast gr