不同辐射剂量损伤后T淋巴细胞亚群%2cTH1和TH2变化观察.pdfVIP

不同辐射剂量损伤后T淋巴细胞亚群，TH1和TH2变化观察 涂序珉 郑 辉 甄 荣 张志兴 中国疾病预防控制中心辐射防护与核安全医学所，北京，100088 目的 观察不同辐射剂量损伤对小鼠T淋巴细胞功能亚群的影响，探讨辐射所致的免疫系统损伤在细胞学和分子水 平上的机制。方法 C57BL/6j小鼠采用60Coγ射线照射诱导辐射损伤模型。总照射剂量分别为0.7、1.4、2.8、和5.6Gy。应用 细胞表面标志和细胞内因子标记，流式细胞仪检测分析不同剂量组小鼠脾T淋巴细胞功能亚群CD3，CD4，CD8改变及Th1 和Th2的变化。结果 1.小鼠脾T-淋巴细胞功能亚群CD3，CD4和CD8在照射后均有明显降低，降低幅度与受照剂量明显相关。 2.CD4/CD8比值显示照射后均有明显升高。3.照射后Th1和Th2均有明显降低，其中Th1降低明显高于Th2。受照剂量大于 2.8Gy组Th2/Th1比值较空白对照组明显升高。结论 不同照射剂量对T淋巴细胞功能亚群CD3，CD4，CD8和CD4/CD8有明显 影响，Th1和Th2和Th2/Th1功能亚群的失衡在辐射所致的免疫损伤中具有较重要作用。 ToobservethechangofT-lymphocytesubsetsCD3,CD4,CD8,Th1 andTh2AfterDistinctdoseofIrradiation TUXu-minZHENGHuiZHENRong NationalInstituteforRadiologicalProtection,ChineseCenterforDiseaseControlandPrevention,Beijing 100088,China ObjctiveToobservetheeffectsofT-lymphocytesubsetsCD3,CD4,CD8andCD4/CD8after differentdoseofirradiation.Toinvestigatethecellularandmolecularmechanismofimmunesysteminjury inducedbyirradiation.MethodsTheC57BL/6jmiceweredividedintonormalcontrolgroupandmodelgroups. Themiceinradiationhurtsmodelwereinducedby60Cogammarays (0.7,1.4,2.8and5.6Gy).Thesurface markerandintracellularcytokinesoflymphocyteswerestainedwithfluorescencelabeledmonoclonalantibodies. ThechangesofT-lymphocytesubsetsCD3,CD4,CD8,IFN-randinterleukin-4inspleencelleswereanalyzedby flowcytometryResults1.TheT-lymphocytesubsetsCD3,CD4,CD8havedistinctreducedafterirradiation.This changerangeisrelationwithgammaraysdose.2.TheCD4/CD8issignificantincreased.3.TheTh1andTh2have thedepressionafterirradiationandthedepressionlevelofTh1ishigherthanTh2.Whenirradiationdoseisover 2.8Gy,Th2/Th1showamarkedincreasedcomparativewithcontrolgroup.ConclusionItissuggestedthatthe changesofmiceT-lymphocytesubsetsCD3,CD4,CD8andCD4/CD8ratiobecausedbydifferentdoseafter irradiation,especiallytheimbalanceofTh1,Th2andTh2/Th1ratioplaysanimportantroleinradiationimmune harm.