肥胖人痰湿体质外周血基因表达谱特征的研究.pdfVIP

中医杂志 2006 年 11 月第 47 卷第 11 期 8·51 · 证 的 研 究 肥胖人痰湿体质外周血基因表达谱特征研究 1 1 2 王　琦 　龚海洋 　高京宏 摘要　 目的: 采用基因芯片技术对痰湿型体质人的外周血基因表达谱进行研究。方法: 挑选 3 例非痰湿型体质肥胖人、2 例痰 湿型体质肥胖人和 3 例正常体重者, 取上述受试者的外周血, 提取总RNA 并进行扩增和标记, 检测受试样品的质量, 进行基 因表达谱观察, 从基因序列中查出具有显著统计学意义的序列所标志的基因。结果: 痰湿型体质与非痰湿型体质相比, 有 115 个差异表达探针组。结论: 本研究发现痰湿型体质人的外周血相关基因表达与非痰湿型体质人有明显差异。 主题词　肥胖症遗传学　痰湿遗传学　@ 基因表达谱 - Study on Character istics of Per iphera l Blood Gene Expression Prof ile in the Obesity w ith Phlegm Dam pness Con stitution 1 1 2 W ang Q i , Gong H aiyang , Gao J inghong : Objective To study on peripheral blood gene exp ression p rofile in the obesity w ith ph legm dampness constitution by gene ch ip . : , 2 , 3 technique M ethods T h ree cases of non ph legm dampness constitution cases of ph legm dampness constitution and norm al persons w ere selected and the extracted total RNA w as m agnified and labeled. T hen the quality of samp les w as detected and the . gene exp ression p rofile w as investigated T he genes m arked by the sequence w ith statistically significant difference w ere searched . : , out from Gene Bank Results A s the ph legm dampness constitution compared w ith the non ph legm dampness constitution there are 115 p robe group s of differential exp