本科植物细胞与基因工程研究型实验课程的构建与实践.pdfVIP

Hereditas (Beijing) 2015 年 4 月, 37(4): 402 ―406 遗传学教学 本科植物细胞与基因工程研究型实验课程的构建与实践 林晓飞，征荣，莫日根 内蒙古大学生命科学学院，呼和浩特 010021 摘要 : 在本科实验教学中开设研究型综合性实验课程是培养创新性人才的重要教改内容。作者利用自身的科研 平台，为本科生开设了植物细胞与基因工程的专业选修课，主要讲授植物组织培养、原生质体分离与培养、植 物遗传转化及转基因植物的筛选与鉴定等植物细胞与分子生物学技术原理及实验操作。通过整合植物组织、细 胞和分子3 个水平的实验教学内容和操作技术，采用集中讲解—独立操作—全天候开放的实验教学模式，并在 实验教学中增加自主设计环节，培养本科生的综合性实验设计能力，训练独立操作技能，激发科研兴趣和自主 创新意识，从而达到培养本科创新人才的目标。文章总结了开展植物细胞与基因工程实验教学的经验和教学效 果，介绍了该实验教学的教学模式、教学内容和方法、考核评价体系，分析了存在的问题，阐述了以高水平科 学研究促进实验教学对于深化本科教学改革和培养现代生物技术创新人才的重要性。 关键词 : 植物细胞工程; 基因工程; 研究型实验教学; 创新人才 Research-oriented experimental course of plant cell and gene engi- neering for undergraduates Xiaofei Lin, Rong Zheng, Morigen School of Life Sciences, Inner Mongolia University, Hohhot 010021, China Abstract: Research-oriented comprehensive experimental course for undergraduates is an important part for their training of innovation. We established an optional course of plant cell and gene engineering for undergraduates using our research platform. The course is designed to study the cellular and molecular basis and experimental techniques for plant tissue culture, isolation and culture of protoplast, genetic transformation, and screening and identification of transgenic plants. To develop undergraduates’ ability in experimental design and operation, and inspire their interest in scientific research and innovation consciousness, we integrated experimental teaching and practice in plant genetic engineering on the tissue, cellular, and molecular levels. Students in the course practiced an experimental teaching model featured by two-week teaching of principles, independent experimental design and bench work, and ready-to-access laboratory. In this paper, we describe the contents, methods, evaluation system and a few issues to be solved in this course, as well as the general