红景天线粒体nad7基因内含子2序列测定与其系统发育意义.pdfVIP

HEREDITAS (Beijing) 2007 3 , 29(3): 371―375 ISSN 0253-9772 研究报告 DOI: 10.1360/yc-007-0371 nad7 2 邓科君, 杨足君, 刘成, 赵为, 刘畅, 冯娟, 任正隆 , 610054 : 开展濒危藏药红景天的分子生物学研究, 可以为红景天遗传资源鉴定与保护提供指导。利用线粒体中 nad7 基因的保守序列设计的引物, 对分布于四川的大花红景天、长鞭红景天和吉林的高山红景天等9 份材料进行 PCR 分析, 发现在所有的红景天材料中均获得约800 bp 的扩增产物。进一步对扩增片段进行序列测定, 结果表明 该片段除具有53 bp 的外显子区外, 其内含子长度均为738 bp, 有9 个核苷酸变异位点。将测定序列进行比对和 聚类分析表明, 红景天线粒体nad7 内含子2 序列被单独聚类。而且测定的红景天的nad7 内含子 2 序列, 其长度 均小于其他高等植物的同源序列, 体现了红景天属植物的该基因区域的特异性, 可能与特殊的生态环境有关。 : 红景天; 线粒体; nad7 基因; 内含子 Identification and phylogenetic application of unique nucleotide se- quence of nad7 intron2 in Rhodiola (Crassulaceae) species DENG Ke-Jun, YANG Zu-Jun, LIU Cheng, ZHAO Wei, LIU Chang, FENG Juan, REN Zheng-Long School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China Abstract: Genetic characterization of 9 populations of Rhodiola crenulata, R. fastigiata and R. sachalinensis (Crassu- laceae) species from Sichuan and Jilin Provinces of China, was investigated using the conserved primer of nad7 intron 2. All PCR products about 800 bp long were shorter than other Crassulaceae plants, which were used as molecular markers to identify the Rhodiola species. The sequence of the products indicated that total exon of 53 bp and intron of 738 bp exhibit only 9 nucleotide variations. Blasting the nad7 sequences to GenBank and the phylogenetic analysis showed that the se- quence of Rhodiola species was clusted independently, and the length was smaller than all the registered sequences of higher plants. The result suggests that the Rhiodola species had a unique sequence in