大黄素抑制人脐静脉内皮细胞TLR4及ICAM-1和表达.pdfVIP

大黄素抑制人脐静脉内皮细胞TLR4 及ICAM-1 的表达 张辉，李昂，段美丽 首都医科大学附属北京友谊医院重症医学科，北京，100050 摘要：为探索大黄素对脐静脉内皮细胞的保护作用。脐静脉内皮细胞 分为对照组、LPS 组、不同浓度大黄素处理组5 个组，采用RT-PCR 测定TLR4 及ICAM-1 mRNA 水平，采用Western-blot 方法测定脐静 脉内皮细胞对于 TLR4 及 ICAM-1 蛋白的表达水平。结果显示， 5µg/ml ，10µg/ml 的大黄素可下调 LPS 诱导的人脐静脉内皮细胞 TLR4、ICAM-1mRNA 及蛋白水平的表达。结果表明，大黄素可能通 过抑制TLR4 诱导人脐静脉内皮细胞ICAM-1 的表达，从而可能减轻 局部炎症反应。 关键词：LPS ；人脐静脉内皮细胞；TLR4 ；ICAM-1 ；大黄素。 Emodin Ameliorates Expression of TLR4 and ICAM-1 in Human Umbilical Venous Endothelial Cells Hui Zhang, Mei-Li Duan, Ang Li Department of Critical Care Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China Abstract: Reducing the expression of Toll-like receptor 4(TLR4) and intracellular adhesion molecules-1 (ICAM-1) is known to decrease inflammation-induced vascular complications. In this paper we looked at whether emodin can reduce inflammation-induced TLR4 and ICAM-1 expression after lipopolysaccharide (LPS) treatment in endothelial cells. Human umbilical vein endothelial cells (HUVEC) were pretreated with 2μg/ml LPS and subsequently exposed to different concentrations of emodin. RT-PCR analysis was used to measure the mRNA expression of TLR4 and ICAM-1. Western blot assay was used to measure the expression of TLR4 and ICAM-1. TLR4 and ICAM-1 appeared to be the targets that were affected by the emodin, with their expressions being partially and almost completely reduced by emodin at 5 and 10μg/ml concentrations in HUVEC. This study showed that emodin reduced TLR-4 and ICAM-1 expression in HUVEC exposed to LPS. Key Words: Lipopolysaccharide, Human umbilical vein endothelial cell, Toll-like receptor4, Intracellular adhesion molecules-1, Emodin.