亚临界流体萃取-气相色谱质谱法测定牡蛎中7+种指示性多氯联苯.pdfVIP

第九届全国超临界流体技术学术及应用研讨会论文集 亚临界流体萃取-气相色谱质谱法测定牡蛎 中 7 种指示性多氯联苯 TPF FPT * 贾凯 刘坤 卢杰 韩玉谦P P 中国海洋大学食品科学与工程学院，山东省 青岛市， 266003 本研究探讨了影响亚临界流体萃取牡蛎中 7 种指示性多氯联苯的因素，包括萃取温度、 压力。结果表明，在温度 30℃，压力 3MPa，静态萃取 20min，动态萃取 40min，共溶剂甲醇 添加量 6mL条件下进行萃取。样品中的多氯联苯采用气相色谱-质谱法进行测定。在选定条件 下 7 种多氯联苯的回收率为 84.8%~102.3%，研究还用样品进行验证，结果表明，本研究所建 立的方法可行。 关键词：亚临界流体萃取，多氯联苯，气相色谱质谱，牡蛎 Analysis of Polychlorinated Biphenyls in oyster product by Subcritical R134a Extraction -Gas Chromatography-Mass Spectrometry JIA Kai, LU Jie, DENG Nan,HAN Yu-qian* (Ocean University of China College of food science and engineering, Qingdao, Shandong.266003) Parameters affecting the Subcritical R134a Extraction.of polychlorinated biphenyls (PCBs) from oyster, including temperature, pressure . The optimal conditions were extraction pressure of 3 MPa at 30 ℃, the static extraction time was 20 min , the dynamic extraction time was 40 min , the modifier was 6 mL methanol. The PCBs in the extract were quantified using gaschromatography-mass spectrometry( GC-MS) The recoveries of seven PCBs ranged from 84.8% to 102.3%. This method was also used to analyze sea products such as oyster and shrimp. The results proved that the established method is reliable. Key words ：Subcritical R134a Extraction, Polychlorinated Biphenyls, Chromatog