Percoll梯度离心前后精子功能指标分析_林海萍.pdfVIP

·161 · 6 　3 　　　　 Vol.6　No.3 　 2000 9 National Journal of Andrology Sep.2000 Percoll 1 1 2 林海萍 , 商学军, 傅　健 (1., 210002; 2., 210093) :　目的:Percoll , 。 　方 法:、-、, 、 。　结果:25 Percoll , (20.56±8.13μm/s 25.18 ±8.30μm/s, P0.05)、(61.20%±21.11% 0.36%±1 .0%, P0.01)(68.28%± 1 .33% 6.6%±1 .10%, P0.05)。 　结论:Percoll , , 。 :Percoll ;;;; :R321.1　　:A　　:1008-3839 (2000)03-0161-03 Analysis of Sperm Functional Parameters before and after Percoll Gradient Centrifugation 1 1 2 Hai-Ping LIN , Xue-Jun SHANG , Jian FU (1.Laboratory of Rep roduction Genetics , Nanj ing General Hosp ital, Nanj ing 210002, China ; 2.D ivision of Cy tobiology , Medical College of Nanj ing University , Nanj ing 210093, China ) Abstract:　Obj ectives :To evaluate the sperm functional parameterswith Percoll gradient centrifugation.　M ethods :The changes of sperm velocity, percentage of acrosomal integrity and percentage of swollen sperm before and after Percoll gradient centrifugation were evaluated by multiple-exposure photography technique, Wright-Giemsa staining and hypo-osmotic swelling test (HOST).　Results :The semen samples from 25 infertile patients were treated by Percoll gradient centrifugation.Before and after the treatment, the changes of sperm velocity (from 20.56±8.13μm/s to 25.18±8.30μm/s;P0.05), percentage of acrosomal integrity (from 61.20%±21.11% to 0.36%±1 .0%; P0.01)and percentage of swollen sperm (from 68.28%±1 .33% to 6.6%±1 .10%;P 0.05)were statistically significant.　 Conclusions :The spermatic functional parameterswere distinctly im