生长抑制和DNA损伤基因45 a在人不同组织类型涎腺肿瘤中的表达.pdfVIP

医学分子生物学杂志,2015, 12 (1):7-11　 J Med Mol Biol,2015, 12 (1):7-11 DOI: 10．3870/ j．issn．1672-8009．2015．01．002 Expression of Growth Arrest and DNA Damage-Inducible45a Gene in Different Types of Human Oral Salivary Gland Tumors 1 2 2 2 2 1 2 GUOShurong , DONGGang ,LITao ,ZHENGJianjin , QI Fangmei ,XUYan ,LUShulai 1Department of Stomatological Centerof QingdaoMunicipalHospital, theAffiliatedHospitalof Qing- dao University, Qingdao, Shandong,266011, China 2Department of Stomatological Center of Qingdao Municipal Hospital, Qingdao, Shandong, 266011, China 【Abstract】 　 Objective　 To investigate the expression of Growth arrest and DNA damage-induc- ible45a ( Gadd45a ) mRNA in normal, benign, and malignant tissues of salivary gland and its pathological significance．Methods　 30 cases of salivary gland tumors from human and the same number of salivary gland tissues adjacent to the tumors were used in this study．The changes of Gadd45a mRNA expression level were detected by reverse transcription-polymerase chain reaction (RT-PCR) followed by Real-time PCR．Results　 Compared to adjacent salivary gland tissues, the Gadd45a mRNA expression in malignant tumors were decreased significantly, the Gadd45a mRNA expression in pleomorphic adenoma and basal cell adenoma were decreased too; But the Gadd45a mRNA expression in warthin tumors were no significant changes．The Gadd45a mRNA expression were lower in malignant salivary gland tumorsthan that in benign (pleomorphic adenoma and basal cell adenoma) salivaryglandtumors (P0．05). Conclusions　 Gadd45amRNAexpressionlevel is decreasedinsalivary glandtumors, whichmayplay animportantroleinthetumorigenesisof sali- vary gland tumors by inducing the tumors continue to proliferation． 【Key words】 　 Gadd45a; salivary gland tumors; RT-PCR 生长抑制和DNA损伤基因45a在人不同组织类型涎腺肿瘤中 的表达 1 2 2 2 2 1