乳香-没药配伍前后化学成分变化及其对LPS-诱导的巨噬细胞产生NO+的影响.pdfVIP

Analyzing and evaluating the changes of chemical components and their effects on the NO level of LPS induced macrophage cell from frankincense-myrrh herbal pair Chen Ting, Su Shulan*, Duan Jin-ao, Shang Erxin, Qian Dawei, Tang Yuping (Jiangsu Key laboratory for TCM formulae Research, Nanjing University of Chinese Medicine, Jiangsu Nanjing 210046) Abstract: Objective: To analyze the differences of chemical constitutes between mixed decoction and co-decoction of frankincense-myrrh and evaluate their effects on the NO level of LPS induced macrophage cell. Methods: The UPLC/Q-TOFMS/MS was used to analyze and identify the chemical components of frankincense-myrrh herbal pair; the MarkerLynx method was adopted to identify the different compounds. Results: The results of PCA showed that there is significant difference between mixed decoction and co-decoction of frankincense-myrrh and the change compounds of pentacyclic triterpenoid (α-boswellic acid, β-boswellic acid) and tetracyclic triterpenoid (elemonic acid, 3-acetoxy-16- hydroxy -dammar-24-ene, 3-hydroxytirucalla-8,24-dien-21-oic acid or 3-hydroxytirucalla-7,24-dien-21-oic acid) were identified as the most changed components based on OPLS-DA. Conclusion: These data may be provide a scientific foundation for deeply investigating the material basis of frankincense-myrrh synergy. Keywords: frankincense-myrrh; the herbal pair; chemical components; macrophage cell; NO 药对是历代医家长期医疗实践的经验总结，组成简单却具备配伍的基本特征，体现了 中医遣方用药的特色优势，具有内在的组合变化规律与丰富奥妙的科学内涵，也是复杂方剂 组成的基础。药对是连接中药和方剂的重要桥梁，又具有组成结构简单、配伍取效特点明确、 宜于开展科学研究等优势。药对的组成具有一定的规律性，其组成方式是两药药性在某种程 度上的吻合与制约，如相须配对、相使配对、相畏配对、寒热配对等，具有丰富的内容和奥 妙的科学内涵[1] 。 药对配伍组合的结构特点、配伍效应及其物质基础等研究是方剂配伍研究的基本单元 2 和重要支撑，对于揭示方剂配伍规律及其科学内涵具有引导价值和点面