山楂麦芽颗粒对侧脑室注射瘦素致大鼠厌食症的影响.pdfVIP

group(High-dosage and Low-dosage).Each group has 12 rats. Except for Normal group, the others were injected with leptin in lateral ventricle for three times everry other day. Meanwhile, Blank group ,Control group and Intervention group are intragastrically administrated with normal saline, Xiang Sha liu jun zi decoction,Chi de xiang decoction(high-dosage and low dosage) in respectively for seven days.At the end of the eighth day, all the rats are sacarificed by intraperitoneal anesthesia.The changes of rats weight were documented everyday.The changes of pathomorphism in gastric and hypothalamus were assayed by HE stains.Results:The weight in normal group is on the increment.However, the weight in other groups are on the decline.There is a significant difference between normal group and other groups.There is a sign that the amount of peptic cells such as chief cell and acid cell are decreased.The high-dosage Chi de xiang decoction can improve that changes,which is similar to the Control group.However, the low-dosage Chi de xiang decoction has no effect in improving that changes. There is no obvious change in hypothalamus .Conclusions:An anorexia nervosa model is successfully established by injecting leptin in lateral ventricle every other day.That model is deserved popularize and application. Key words:leptin; injecting leptin in lateral ventricle;anorexia nervosa; 摄食障碍（eating disorders，EDS）以严重异常的进食行为为特征，可分为神经性厌食症 （anorexia nervosa，AN）、神经性贪食症（bulimia nervosa，BN）、不典型摄食障碍（atypital eating disorder）和暴食症（binge eating disorder，BED）【1】。EDS 多见于青少年，女性居多， 男性发病较少，流行病学资料显示普通人群中患病率约0.5%∼3%，男性仅占0.5%【2】。过往关于EDS 的研究集中在环境、心理等因素为主，近年来随着对摄食基因网络调控研究深入，摄食-能量-代 谢平衡已成为研究热点。本实验通过大鼠侧脑室隔日注射瘦素制备 EDS 模型，目的是为 EDS 的病 理生理及药理研究提供动物模型，并观察受试药山楂麦芽颗粒的干预作用。 1 材 料 1.1 试剂与仪器 Leptin 购自Sigma-aldrich公司（货号L5037，1mg，lot077k1283），将其溶于2mL生理盐水， 并分装成 200μL 小包装，-40℃保存。阿奇霉素颗粒剂（商品名希舒美 0.1×6 包），购自广州中医