甲醛值滴定法快速测定牛奶中蛋白质含量.pdfVIP

中国食品卫生杂志 　　　 　　　　　 —244 — 2008 年第 20 卷第 3 期 CHINESE JOURNAL OF FOOD HYGIENE 实验技术与方法 甲醛值滴定法快速测定牛奶中蛋白质含量 田志梅 (河北省衡水市疾病预防控制中心 ,河北 　衡水 　053000) 摘 　要 : 目的　建立牛奶中蛋白质的快速检验方法 - 甲醛值滴定法 。方法 　利用氨基酸的两性特性 ,加入甲醛溶 液并用标准碱溶液滴定 ,通过测定牛奶中蛋白质的游离氨基酸含量 ,计算求得牛奶中蛋白质含量 。结果 　牛奶中 蛋白质含量与游离氨基酸含量呈良好的正相关 , RSD % 263～340 ,本法与国标法两种方法测定结果的绝对差值 与其算术平均值之比小于 5 % ,测定时间由约 3 h 缩短为 3～5 min 。结论 　本方法有效地解决了非蛋白质类的含氮 物质对牛奶中蛋白质测定结果的干扰问题 ,适用于牛奶与奶粉中蛋白质的快速定量检验 。 关键词 :乳 ;蛋白质类 ; 甲醛 ;滴定分析法 Determination of Protein Content in Milk by Formaldehyde Value Titration TIAN Zhimei ( Hengshui Municipal Center for Disease Control and Prevention , Hebei Hengshui 053000 , China) Abstract : Objective 　To determine protein content in milk by formaldehyde value titration. Method 　Using amphoteric property of amino acids , the formaldehyde solution was added into milk sample , and then the sample was titrated by standard alkali. The protein content in milk was calculated by determining free amino acids of it. Results 　There was a good correlation between protein content in milk and free amino acids. The RSD was 263 %～340 %. Compared with the national standard method , the ratios of absolute difference to its arithmetic average of two methods were all below 5 %. The determining time was 3 ～5 min , and less than 3 h of national standard method. Conclusion 　The method could effectively resolve the interference problems of nitrogen substances which belong to nonprotein kinds , and apply to rapid quantitative test of pr