眼柄粗提物对凡纳滨对虾性分化的影响.pdfVIP

第 31 卷 第 1 期 广东海洋大学学报 Vol.31 No.1 2011 年 2 月 Journal of Guangdong Ocean University Feb. 2011 眼柄粗提物对凡纳滨对虾性分化的影响 朱春华，赵光凤，李广丽 （广东海洋大学水产学院，广东 湛江 524025 ） 摘 要：采用生化方法制备凡纳滨对虾[Litopenaeus Penaeus vannamei ）]眼柄粗提物，用活体注射法研究不同粗 提物剂量对去眼柄凡纳滨对虾卵母细胞直径大小的影响，以检验其性腺抑制素（Gonadinhibiting hormone , GIH）的 生物活性；在凡纳滨对虾仔虾后 17、22 和 27 d，分别以不同浓度眼柄粗提物浸泡 24 h，结果显示：除 0.001 个眼 柄/mL 的 GIH 浓度外， 0.01、0.1 和 1 个眼柄/mL 组均显著降低凡纳滨对虾卵母细胞直径（P ﹤0.05）；浸泡处理后， 仔虾后 17 d GIH 处理组凡纳滨对虾雌性率达 70.1%～80.1%；仔虾后 22 d GIH 处理组雌性率达 65.8%～71.7%，各 浓度 GIH 处理组雌性率比对照组（雌雄比例约为 1∶1）显著提高（P ﹤0.05）；仔虾后 27 d，0.01 和 1 个眼柄/mL 处理组雌性率 （约 58%）略高于对照组，但 0.1 个眼柄/mL 处理组雌性率 （73.1%）与对照组相比显著提高 （P ﹤0.05）。 结果表明较低浓度的眼柄粗提物可明显诱导凡纳滨对虾雌性化，在仔虾后 22 d 前处理效果最佳。 关键词：凡纳滨对虾；眼柄粗提物；性分化；性腺抑制素（GIH ）；雄性腺激素（AGH ） 中图分类号：Q942 + Q959.223+ .63 文献标志码：A 文章编号：1673-9159 （2011 ）01-0023-05 Effects of Eyestalk Extracts on the Sex Differentiation on Litopenaeus vannamei ZHU Chun-hua, ZHAO Guang-feng, LI Guang-li （Fisheries College, Guangdong Ocean University, Zhanjiang 524025, China） Abstract ：The eyestalk extracts were obtained by biochemistry methods and their activities of gonad-inhibiting hormone GIH were checked by vivo injection 0.001, 0.01, 0.1, and 1 eyestalk per mL on female Litopenaeus vannamei . The shrimps were immersed in different doses of eyestalk extracts 0.01, 0.1 for 24 hours, and 1 eyestalk per mL at 17, 22 and 27 days after postlarva, respectively, and the effects of GIH on the sex differentiation were investigated. The results showed that strong GIH activities existed in the eyestalk extracts solution 0.01 - 1 eyestalk/mL except that of 0.001 eyestalk/mL, and the oocyte diameter decreased significantly treated by the eyestalk extracts P ﹤0.05 . Female rate reached to 70.1% - 80.1% in the group treated at postlarva for 17 days, and 65.8% - 71.7% at postlarva for 22 days. There was significant difference in female rate between the control and the eyestalk extracts groups P ﹤0.05 . However, there was no significant difference