何首乌化学成分分离及其提取物微生物发酵前后成分比较的研究.pdf

Isolation and chemical constituents of Polygonum multiflorum extract Comparison of composition before and after fermentation Speciality：Pharmaceutical Chemistry Graduate ：Wang Zhen Tutor ：Prof ．Zhou Honglei Abstract Polygonum commonly used in clinical medicine.The drug is the dry root of Polygonum plants, distributed in the Northeast, South, East, Southwest provinces. Modern research found that Polygonum contain anthraquinone, stilbene glycosides, flavonoids and other ingredients, it can promote hematopoietic function, enhanced immune function, lowering blood pressure and anti-atherosclerosis, anti-tumor, hepatoprotective, anti-aging, endocrine, laxative and so on. After processing of traditional Chinese medicine，the quality and quantity of the internal components were changed, it would better serve the advantages of traditional Chinese medicine. Fermentation is one important way of processing of traditional Chinese medicine, as one of the important methods of processing, has received more and more attention of the world. In this paper,12 compounds were isolated from Polygonum identified and 11 compounds were identified as β-sitosterol ( Ⅰ)physcion ( Ⅱ) , p-formaldehyde (Ⅲ), emodin ( Ⅳ), emodin -8-- A ether ( Ⅴ), ω-hydroxy-emodin ( Ⅵ), emodin-8-O- β -D-glucopyranoside ( Ⅶ ), emodin-8-O- β -D-glucopyranoside ( Ⅷ ), 2,3,5,4 ′ -tetrahydroxysilbene -2-O- β-D-glucopyranoside (Ⅸ), D-glucose ( Ⅹ), D-mannotol （Ⅺ）, in which D-mannitol (Ⅺ) for the first time to get from the herbs. In order to compare the change of pharmacological efficacy and chemical composition,we first optimized by orthogonal experiments Polygonum decoction extract the best conditions.Compare the acute tox