大孔树脂纯化肺形草总黄酮工艺研究.pdfVIP

· 94 · ChineseJournaIof lnformat；on OnTCM Mar．2015VoI．22No．3 大孔树脂纯化肺形草总黄酮工艺研究 袁桥玉 ’，刘新桥 1．武汉职业技术学院生物工程学院，湖北 武汉 430074；2．中南民族大学药学院，湖北 武汉 430074 摘要 ：目的 研究大孔树脂分离纯化肺形草中总黄酮的工艺。方法 通过静态吸附及解吸试验，以吸附率 和解吸率为指标考察 6种型号大孔吸附树脂对肺形草中总黄酮的纯化性能，筛选最佳的大孔树脂，采用动态吸 附考察上样液浓度、上样流速、上样量对吸附的影响，并通过正交试验确定最佳洗脱工艺。结果 AB一8型大孔 树脂对肺形草总黄酮有较好的吸附和洗脱效果，其最佳分离纯化工艺为：上样液浓度为5．285mg／mL，上样流速 为2BV／h，上样量为 17．62mg／mL，依次用4BVl0％乙醇洗脱除杂，5BY50％乙醇洗脱总黄酮，洗脱流速为 4BV／h。 经AB一8树脂处理后的总黄酮纯度达61．95％，收率为87．28％。结论 该优选工艺稳定可行，适用于肺形草总黄酮 的纯化分离。 关键词：肺形草；大孔吸附树脂；总黄酮；纯化工艺 D0I：10．3969／j．issn．1005—5304．2015．03．025 中图分类号：R284．2 文献标识码 ：A 文章编号 ：1005-5304(2015)03—0094—04 Purification ofTotalFlavonoidsfrom TripterospermiChinensisHerba by M acroporous Resin YUANQiao—yu，LIUXin-qiao f1．Schoolo／Bioengineering,WuhanPolytechnic,Wuhan430074,China： 2．SchoolofPharmaceuticalSciences,South—CentralUniversityyorNationalities．Wuhan430074,Chin口J Abstract：OblectiveTo optimizethepurification technologyoftotalflavonoidsfrom the TripterospermiChinensisHerbabym acroporousresin．MethodsThepurification abilitiesofsix kindsofm acroporousadsorption resinsfortotalflavonoidsfrom Tripterosperm iChinensisHerba were studied with theadsorption and desopr tion ratesasthe index by staticadsorption and desorptionexperiments．Thecolum nliquidconcentration，adsorptionrate，andtheloadedamount werestudiedbyusingdynamicadsorption experiments．Theoptimaldesorption technoloyg was examined by orthogonalexperim ent．ResultsAB-8 macroporousresin wasfound to have good adsorptionanddesorptioneffects．Theoptimalpurification conditionswereasfollows：thecolumn liquidconcentrationwas5．285mg／mL．adsorptionratewas2BY／h；theloadedamountwas 17．62mg／mL．Thesamplewaselutedby10％ethanolwith4BVand50％ethanolof5BVataflow rateof4BV／h．Thepurityoftotalflavonoidsincreasedto61．95％afterthepurification，andthe yield was 87．28％．Conclusion This optim ized pr