APP17肽对糖尿病小鼠学习记忆功能和海马NT_3_胆碱乙酰化酶神经元的影响.pdfVIP

1999 12 18 6 Chin J Geriat r, D ec . 1999, V ol. 18, N o . 6 349 A P P 17 -3 N T 钱玉英　赵咏梅　姬志娟　赵志炜　盛树力 N T -3 , AP P 17 ( AP P3 19335) ( , ) , 17 , 4 ; Strepto zo to cin ST Z A PP , ( 1) A PP 17 , ( 29. 213. 52) ( 28. 9 13 . 2 1) mmol L , ( P 0 . 01) ; ( 2) : A P P 17 A P P 17 , ; ( 3) : AP P 17 NT -3 ( 90. 4 115 . 42) ( 88. 75 4 . 16) ( 56 . 254 . 95) ( 60. 925. 47) , ( 6 1. 7113 . 4 1) ( 89 . 4 813. 48) ( P 0 . 0 1) , A PP 17 ( 10. 313 . 7 1) ( 12. 24 3. 28) ( 82. 40 17 . 74) ( 79 . 40 15 . 34) , ( 5 . 240 . 87) ( 106. 38 10. 76) ( P 0 . 05) A P P 17 , -3 N T The effect of APP17 peptide on the function of learning andmemory andNT-3choline acetyltransferase neuron of hippocampus in diabetic mice QIA N Y uying , ZH A O Yong mei, J I Zh ij uan , et al . B eij ing R esearch L abor atory or B rain A g ing , B ej ing X uanw u H osp ital , B eij ing 100053 Abstract Objective T o observe the function of learning and memory and the immuno histo chemical chang e of hippo campus in diabetic mice and the prot ective effects of A PP 17 peptide fro m diabetes. MethodsM ouse diabetic model w as produced with streptozoto cin and A PP 17 peptide as a pro tective ag ent w as injected subcutaneously int o diabet ic mice. Four weeks later , water maze t est w as per for med. A fter water maze test , fixative w as injected intr avascularly into the mice , brain w as r emoved for pr eparat ion of cr yostat sections, and immunohistochemical stain w as done with neur otro phin-3 ( NT -3) , choline acety ltr ansfer ase antibodies. Results( 1) T he bloo d glucose level in diabetic mice wit h inj ect ion of A PP 17 peptide ( 28. 9 13. 21) mmol L