RNAi介导的SOCS3基因沉默对高脂肥胖大鼠瘦素抵抗的影响.pdfVIP

Med No．8 ·论 善· J 2012，V01．41 Res，Aug 古H匕 RNAi介导的SOCS3基因沉默对同月目 肥胖大鼠瘦素抵抗的影响 张 旭 刘正娟 翟 娜 吴 鸣 滕懿群 陈俊国 骆秋龙 陆 燕 摘要 养8周末，检测血总胆固醇、三酰甘油、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇，放射免疫法检测血清瘦素，利用荧光实时定 量PCR(real—time 0．05)。结论下调SOCS3基因可以减轻高脂肥胖大鼠瘦素抵抗，对肥胖症可能有预防和治疗作用。 关键词 肥胖RNA干扰瘦素抵抗瘦素受体细胞因子信号转导抑制因子3 EffectsofRNAi—mediatedSOCS3GeneSilenceon ResistanceinObeseRatsFedwith Diet． Leptin High—fat ZhangXu，LiuZhengjuan， Zhai Second Na，WuMing，Teng Qiulong，Luran．DepartmentofPediatric，TheHospitalofJiaxingMunicipal， Yiqun，ChenJunguo，Luo 314000，China Zhejiang Abstract To theeffectsofRNAi—mediatedSOCS3silenceon resistanceinobeseratsfedwith 0bjective study gene leptin high —fitdiet．Methodsfive—week—oldmaleSDratsweredividedintothree Sixty healthy groupsrandomly．Inexperimentgroup．the lentiviral SOCS3 intothe vector a RNA expressingshort—hairpintargeting gene(LV—shSOCS3)werestereotaxicallyinjected hypothala— musofratswhichthenwith diet．Inthe lentiviralvector a RNA anan— fed high—fat negativegroup．the expressingshort—hairpintargeting related intothe ofratswhichthenfedwith thecontrol sequence(LV—negtive)werestereotaxicallyinjected hypothalamus high—fatdiet；In of end normalsodiumwere intothe ratswhichthenfedwith diet．Atthe