利用巢式PC R检测花生青枯病菌.pdfVIP

Fu ian Journal o A r icultural Sc iences j f g PC R J DIN G XL Y O U Y H U A N G JC et al Det ectio n of R als tonia so lanace ar um of Peanut by a N est ed PC R J Fuj ian Jo urnal of A g r ic ultur al Sc iences PC R SS rDNA IT S L L DNA W W L L PCR W W bp PCR fg L DNA PCR PCR S A Detection of Ralstonia solanacearum of Peanut by a Nested PCR DING Xueling YOU Yong H U A NG Jiancheng LIU Pei ing C HEN Qinghe XIE Shi ong q y I ns titute o Plant P rotection Fu ian A cadem o A r icultural Sc iences K e L abo rator o I nte rate d Pes t f j y f g y y f g M ana ement o r Fu ianTaiw an Cro s M inis tr o A r iculture Fuz hou Fu ian China g f j p y f g j Abstract T o develop a technique for the quick detection of R als tonia solanacearum of Peanut a nested PCR w as established Bacterial universal primer pair L L based on the intergenic transcribed spacer ITS region of S S ribosomal DNA of bacteria w as used as the first round primers and the PCR products w ere cloned and sequenced A pair of special PCR primers WW w as designed as the second round primers T he prime pair could amplify a single bp band from genomic DNA of R alstonia solanacearum in peanut but not f rom other bacteria T he sensibility of nested PCR reached fg L of DNA times higher than that of a sample PCR Using nested PCR assay the pathogen could be specifically detected from