沙棘叶总黄酮的提取纯化方法概况.pdfVIP

· 研 究 进 展 Western Journal of Traditional Chinese Medicine,2015 Vol.28 No.3 沙棘叶总黄酮的提取纯化方法概况 * △ 曾圣雅，周吉银 第三军医大学第二附属医院国家药物临床试验机构，重庆 400037 ［摘 要］查阅国内外有关文献探讨从沙棘叶中提取纯化总黄酮的方法，指出提取方法有回流提取法、微 波萃取法、普通超声提取法、循环超声提取法、微波超声双辅助提取法，其中回流提取率较高，微波法次之，但 超声法和超声循环提取法可节约时间和能耗，效率也较高；纯化萃取方法有酸提碱沉法、膜过滤法、大孔吸附 树脂法、聚酰胺洗脱法，其中优选膜过滤法和大孔树脂吸附法纯化后总黄酮含量较高且方法简便可行，成本较 低。 虽然对沙棘总黄酮的提取纯化方法的研究取得了一定进展，但还需摸索更有效的方法，并且需进一步提高 提取率和萃取后总黄酮的含量。 ［关键词］沙棘叶；总黄酮；提取纯化 ［中图分类号］R284.2 ［文献标识码］A ［文章编号］1004-6852(2015)03-0136-04 A Survey on the Extracting and Purifying Methods of Total Flavonoids in Seabuckthorn Leaves ZENG Shengya, ZHOU Jiyin △ National Drug Clinical Trial Institution of The Second Affiliated Hospital of Third Military Medical University, Chongqing 400037, China The pertinent documents at home and abroad were consulted to survey the extracting and purifying methods for total flavonoids from seabuckthorn leaves. The main extraction methods concluded were refluxing method, conventional ultrasonic method, circulating ultrasonic method, microwave method as well as microwave and ultrasonic auxiliary method, among which the refluxing method was with better extracting rate and the next was microwave method, but the ultrasonic method and circulating ultrasonic method can reduce time and energy with good efficacy. The methods for the purification included alkali extraction and acid precipitation method, membrane filtration method, macroporous resin adsorption method and polyamide elution method. Among them, the filtratio