CCR9CCL25介导小鼠经生殖道hCGβ避孕疫苗粘膜免疫后B细胞归巢.pdf

嚣蹙壅受￡￡堕笪￡磐』盒昱41基墅生蕉遒埏曼垦羹垄痊苴整壁焦瘥量垒塑照堡墨墨!掇 ·819· ·生殖免疫学· 贺晓菊唐传玲李明清姚晓英李大金o (复旦大学上海医学院附属妇产科医院暨妇产科研究所生殖免疫研究室，上海200011) 中国圈书分类号R169．41文献标识码A 文章编号1000．484X(2007}凹-帕19-06 达亦增高。结论：重组乳酸杆菌活疫苗Ih CCL25可介导B细胞归巢至生殖遵局部。这可能增强hCGl3粘膜避孕疫营的避孕般果。 【关键词】hCGl5；粘膜免疫；CCR9／CCl25；B细胞；归巢 in CCR9／CCL25Bcell atmurine tractaRer engaged homing genital tionvia withlactobacillus vagina expressinghCG[1 HE lmmunolo· Xiao·Ju，TANGChuan-Liog，LIMing-Qing，YAOXioa—H喈，LIDa-Jin．LaboratoryforReproductive Medical 200011， gy，Hospital＆lmtittaeofObstetrics＆母船蝴，FudanUniters蚵StmngtuziCollege，Shanghai China themechanismforBcell in tTactafterinoculationofhctobecilhs investigate [Abstract]Obj酬：T0 homingmurlne目mital micewereinoculated with expressinghc邴viavagi∞．Methods：BALB／c intravaghmlly10”n．pIlac afterthesec- aboostinthree帕．kinterve]．Thetitersof douchetwoweeks by aHtib(嘶in lively．followed antihc印I掣∥IgAY蟛nal immunizationweredeterminedindirect immunosorbem cells ondary enzyme-linked IgG／IgAantibodyproducing by agsay(EUSA)．The in of inroutinetRerine (ASo)levelsalltheimmunizedwereevaluatedwithEUSPoT ehemokine groups assay．Transcriptionreceptors cellsof ofCCI．25inutenlawemin- B CCR9 both删p8were8IIa】p