1型神经纤维瘤病基因表达谱差异的比较研究.pdfVIP

分化与发育、脂类代谢、免疫反应等。结论 NF1 的恶变可能与细胞的增殖与凋亡比例失调有关，趋化因子 CXCL14 表达的缺失，可能与恶性细胞逃避免疫监督有关。 【关键词】 1 型神经纤维瘤病，恶性周围神经鞘瘤，基因芯片，基因，表达谱 Differential GeneExpressionProfileofneurofibromatosistype 1 Differential GeneExpressionProfileofneurofibromatosistype 1 DDiiffffeerreennttiiaallGGeenneeEExxpprreessssiioonnPPrrooffiilleeooffnneeuurrooffiibbrroommaattoossiissttyyppee11 HuXiaogang,ZhangWenjun,ZhangJianlin,JiangHua,YuanXiangbin,LiuAntang,ZhuLie HuXiaogang,ZhangWenjun,ZhangJianlin,JiangHua,YuanXiangbin,LiuAntang,ZhuLie HHuuXXiiaaooggaanngg,,ZZhhaannggWWeennjjuunn,,ZZhhaannggJJiiaannlliinn,,JJiiaannggHHuuaa,,YYuuaannXXiiaannggbbiinn,,LLiiuuAAnnttaanngg,,ZZhhuuLLiiee DepartmentofPlastic and Reconstructive Surgery, SecondMilitary MedicalUniversity, Changzheng Hospital, DepartmentofPlastic and Reconstructive Surgery, SecondMilitary MedicalUniversity, Changzheng Hospital, DDeeppaarrttmmeennttooffPPllaassttiiccaannddRReeccoonnssttrruuccttiivvee SSuurrggeerryy,, SSeeccoonnddMMiilliittaarryy MMeeddiiccaallUUnniivveerrssiittyy,, CChhaannggzzhheennggHHoossppiittaall,, FengYangRoad415,Shanghai,China FengYangRoad415,Shanghai,China FFeennggYYaannggRRooaadd441155,,SShhaanngghhaaii,,CChhiinnaa Abstract Objective Abstract Objective 【 AAbbssttrraacctt】 OObbjjeeccttiivvee To investigate the difference of the gene expression profiles between the dermal neurofibroma and malignant neurofibroma from the same NF1 patient by oligonucleotide microarray analysis and Methods Methods identify the key malignant-related genes. MMeetthhooddss Samples from three NF1 patients who match the diagnostic criteria of the NIH were processed from total RNA and purified to mRNA, which was reverse-transcripted to cDNA. Labeled cRNA was pre