基于光酶诱导药材中次生代谢产物变化研究.pdfVIP

基于光酶诱导药材中次生代谢产物变化的研究 ※ ※ 谷希达 孙铭遥 许科帝 崔磊 陈润泽 张琳 田景奎 浙江大学生物医学工程与仪器科学学院，生物医学工程教育部重点实验室，浙江省心脑血管 神经系统药效筛选与中药开发重点实验室 （杭州 310027 ） 关键词：光酶诱导Chalcomoracin, moracin N, Diels-alder 加合物, 桑叶 摘要：UV 辐射对人类和植物都是有害的。许多植物次生代谢产物，比如黄酮、生物碱、 木质素等，都是紫外吸收物质，能够降低植物受到的损害；这些成分是天然植物药的有效成 分，紫外辐射可以激活具有保护作用的植物次生代谢机制。本论文主要工作是建立了一种从 桑叶中光酶诱导天然活性成分的方法。通过 HPLC 指纹图谱检视，5 个色谱峰在诱导之后产 生了显著的变化。我们鉴定了其中的两个色谱峰所代表的化合物：1.chalcomoracin , 一种具有 抗病毒活性的天然Diels-Alder 加合物；2. moracin N ，是chalcomoracin 的前体物质。照射之 后，他们的含量分别是0.818mg/g 和0.352mg/g 。 Change of Secondary Metabolites In Mulberry Leaves after UV-B Induction Keywords: UV radiation, Chalcomoracin, moracin N, Diels-alder type adducts, mulberry leaves Abstract : Ultraviolet-B ( UV-B) radiation is harmful to plants and humans. Secondary metabolites, like flavonoids, alkaloids, and lignin, are active components of natural medicine. They are also UV-B absorbing compounds, which can shelter plant’s genetic material. Our idea is to utilize harmful effectiveness of UV-B radiation to active plants’self-protection though stimulating secondary metabolism. The results of this paper provide a pathway to induce bioactive products from mulberry leaves in vitro by UV-B irradiation. In the course of radiation, extraction, and separation, five new chromatographic peaks were found by HPLC fingerprint. From which two active compounds were identified, one is chalcomoracin, a natural Diels -Alder type adduct, and the other is moracin N, the precursor of chalcomoracin . Their contents are 8.18mg/10g and 3.52mg/10g in dry weight respectively. Chalcomoracin exhibited considerable antimicrobial activity against methicillin -sensiti