外源糖对苹果果实山梨醇代谢相关酶活性de影响.pdfVIP

天津农业科学 AgriculturalSciences 2011，17(3)：5-8 ·植物生理与生物技术 外源糖对苹果果实山梨醇代谢相关酶活性的影响 叶成荣，刘更森，王永章，李培环，刘成连，原永兵 (青岛农业大学 园林园艺学院，山东 青岛266109) 摘 要：以新红星苹果为试材，采用果实圆片孵育体系研究了外源糖对 SDH和 SOX活性的影响。结果表明：在整个发育过 程中，苹果果实的SDH活性与葡萄糖 、果糖和蔗糖含量间不存在显著的相关性 ；而SOX活性与葡萄糖和果糖含量间存在极 显著的相关性。外源山梨醇和蔗糖均可提高发育前期和发育后期苹果果实的SDH和 SOX活性，其中以山梨醇的诱导作用 最为显著。外源葡萄糖对 SDH活性 的增加具有显著的促进作用，但抑制 SOX活性，而外源果糖对苹果果实SDH和SOX活 性的诱导效果与葡萄糖恰好相反。结果还表明，外源糖对发育前期苹果果实SDH和SOX的诱导作用均显著高于对发育后 期苹果果实的诱导作用。由此推断，苹果果实中山梨醇代谢相关酶的活性变化受到可溶性糖的调控。 关键词 ：苹果果实；外源糖 ；山梨醇脱氢酶；山梨醇氧化酶 中图分类号：$661．1 文献标识码 ：A DOI编码：10．3969~．issn．1006—6500．2011．03．002 EffectsofSolubleSugarsonRelatedEnzymeActivitiesofSorbitolM etabolism inDevelopingAppleFruit YECheng-rong，LIU Geng-sen，WANG Yong-zhang，LIPei-huan，LIU Cheng-lian ，YUAN Yong—bing (CollegeofGardensandHorticulture，QinogaoAgricultureUniversity，QingOao，Shandong266109，China) Abstract：TheregulatingeffectsofsolublesugarsonactivitiesofSDH andSOX werestudiedin S‘tarkrimson’applefruitsthrough diskincubation．TheresultsshowedthattheactivitiesofSDH inapplefurithadnosignificantcorrelationwiththeconcentrationsof glucose，furctoseandsucroseduringthewholedevelopmentalperiod．However，theactivitiesofSOX hadsignificantpositivecolTe— lationwith glucoseandfurctoseconcentrations．Th eresultsalsoindicatedthatexogenoussorbitoland sucrosehighlyincreasedthe activitiesofSDH and SOX，moreover，theexogenousglucoseindicatedthehighestpromotingeffectsonSDH activity，butinhibited theactivityof SOX significantly．Ontheotherhand，theexogenousfurctoseshowedreverseregulatingactiononSDH andSOX ac- tivitiescomparedwithexogenousglucose．Th eexogenousstudyalsoindicatedthattheregulatingeffectsofexogenoussugars onthe furitatearlydevelopmentalstagewere much highercomparedwiththefruitatlatedevelopmentalstage．Itisconcluded thatthe metabolism ofsorbitolinapplefuritwasregulatedbysolublesugars． Keywords：applefurit；exogenoussugar；sorbitoldehydrogenase；sorbitoloxidase 果实所积累糖的种类 、含量及比率是决定其