线粒体途径在慢性低氧高二氧化碳小鼠骨骼肌凋亡中的作用.pdfVIP

J Med No．6 ·论 善· Res，Jun2012，Vol，41 线粒体途径在慢性低氧高二氧化碳小鼠 骨骼肌凋亡中的作用 陈永青 郑 续 邵蒙蒙 王小同 摘要 目的探讨线粒体途径在慢性低氧高二氧化碳小鼠骨骼肌凋亡中的作用。方法成年storagefeature(sPr) protect 过氮气调节舱内氧浓度，使其维持在9％一11％，通入二氧化碳使其浓度维持在5％一6％，每天8h，共4周。用酶联免疫吸附测 一3含量增加(P0．05)，Cyt—CmRNA表达增多，BaxmRNA表达增多，而Bel一2 线粒体途径可能参与了慢性低氧高二氧化碳小鼠骨骼肌细胞的凋亡，Bcl一2／Bax比值下降可能是促进骨骼肌线粒体途径凋亡 的重要因素。 关键词 低氧高二氧化碳细胞凋亡线粒体途径 EffectofMitochondrhtlinThe ofSkeletalMtmelsofTheMice toChronk Chen Pathway Apoptosls Exposed Hypoxia—hypercapnia． 2nd Yongqing，ZhengXu，ShaoMengmeng，WangXiaotong．CenterofNeurology＆Rehabilitatwn，The cal 325000，China College，ghqiang Abstract Toobservetheeffectofmitoehondrialinthe ofskeletalmuscleofthemicewhichwasex- Objective pathwayapoptosis miceof divided tochronic SixteenadultmaleC57BL／6 feature(SPF)levelwere posed hypoxia—hypereapnia．Methods storageprotect intotwo contml the latter inthe closed randomly groups：thegroup(a=8)andexperimentgroup(n=8)．Thegroupw∞placedspecial cabinwhereconcentrationof wasmaintainedwithin9％～1 concentrationofcalhondioxidewas oxygen 1％byinputtingN2and regulated within5％一6％for8hoursa 4 wag the of day，forweeks．Caspase一3proteinassayedbyELISA，andexpressionCyt—CmRNA，Bax was real—timePCR．Results withthecontrol wasmorein— mRNAandBcl一2mRNA by analyzed Compare