电纺纤维膜双重控制释放生长因子及其小口径人工血管的研究.pdfVIP

电纺纤维膜双重控制释放生长因子及小口径人工血管研究 袁晓燕**1张红1贾潇凌2韩凤选1赵蕴慧1赵瑾1樊瑜波**2 1.天津大学材料科学与工程学院，天津市材料复合与功能化重点实验室，天津 300072 2.生物力学与组织工程教育部重点实验室，北京航空航天大学生物与医学工程学院， 北京 100083 小口径人工血管的移植易出现由血栓引起的再狭窄等问题，采用组织工程的方法可以构 建新型生物降解性人工血管，在完善血管内皮化的同时促进自身血管再生[1,2]。电纺纤维膜常 用作人工血管材料，而同轴电纺和乳液电纺可用来负载生物活性物质[3,4]。本文研究了电纺纤 维膜负载和控制释放两种生长因子的方法，即同时负载血管内皮生长因子(VEGF)和血小板衍 生生长因子(PDGF)的方法，为小口径人工血管替代物制备提供新途径。 Dual-deliveryofVEGFandPDGFbyelectrospunmembranesfor small-diameterbloodvesselregeneration YUANXiaoyan1ZHANGHong1JIAXiaoling2HANFengxuan1ZHAOYunhui1 ZHAOJin1FANXubo2 1.SchoolofMaterialsScienceandEngineering,TianjinKeyLaboratoryofCompositeand FunctionalMaterials,TianjinUniversity,Tianjin300072; 2.KeyLaboratoryforBiomechanicsandMechanobiologyofMinistryofEducation,Schoolof BiologicalScienceandMedicalEngineering,BeihangUniversity,Beijing100083 Abstract:Tissueengineeringofsmall-diameterbloodvesselsisstillchallenginginrestenosisand bursting.Topreventthrombopoiesis,rapidendothelializationalongthelumenofgraftsisintended, followedbyproliferationofvascularsmoothmusclecells(VSMCs)aroundtheexteriorfor compliance.Tothisgoal,twomodifiedcoaxialelectrospinningtechniquesweredevelopedto encapsulatevascularendothelialgrowthfactor(VEGF)andplatelet-derivedgrowthfactor-bb (PDGF)respectivelytoregulateproliferationofvascularendothelialcells(VECs)andVSMCs. Releaseprofiles,invitrocellproliferationandinvivoimplantationofdouble-layeredelectrospun membranescomposedofchitosanhydrogel/poly(ethyleneglycol)-b-poly(L-lactide-co-caprolactone) (PELCL)electrospunmembraneloadedwithVEGFastheinnerlayerandemulsion/PELCL electrospunmembrane-loadedPDGFastheouterwereinvestigated.Itwasconcludedthat dual-releaseofVEGFandPDGFcouldaccelerateVECproliferationinthefirst6days,and modulateslowVSMCproliferationintheinitial3daysb