天麻素与葛根素配伍对DPPH自由基清除作用及溶解度的影响姜丽.pdfVIP

( ) • 2397 • 中华中医药杂志 原中国医药学报 20 13年8 月第28卷第8期 CJTCMP , August 20 13, Vol .28, No. 8 ·研究报告· 天麻素与葛根素配伍对DPPH自由基清除作用及 溶解度的影响 姜丽，王玉蓉，林珈好，曹唯仪，黄镇林 （北京中医药大学中药学院，北京 100102） 摘要： 目的：研究天麻素与葛根素配伍后在二苯代苦味肼基自由基（DPPH自由基）清除率、溶解度方面 的相互影响，探讨其配伍应用的合理性。方法：采用紫外分光光度法测定体外DPPH自由基清除率及平衡溶解 度。结果：天麻素浓度为200g/L时，未显示DPPH自由基清除能力，而葛根素5g/L时显示了一定的作用，其IC50为 17.56g/L；天麻素在水中溶解度为303.81g/L，葛根素为1.62g/L，天麻素150g/L时使葛根素的溶解度增加了4.1倍。 结论：天麻素与葛根素配伍后，可增加葛根素溶解度，但在一定浓度范围内对其自由基清除作用无显著性影响。 关键词：天麻素；葛根素；DPPH自由基；清除率；溶解度 基金资助：北京中医药大学复方中药制药研究创新团队（No.2011-CXTD-13） Pilot study on DPPH free radical-scavenging activity and solubility of combining gastrodin with puerarin JIANG Li, WANG Yu-rong, LIN Jia-hao, CAO Wei-yi, HUANG Zhen-lin ( School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China ) Abstract: Objective: Study the influence on DPPH free radical-scavenging activity and solubility of gastrodin and puerarin, to explore the compatibility rationality of the two components. Methods: The ultraviolet spectrophotometry was used to determine the scavenging rate and solubility of gastrodin and puerarin in vitro. Results: Gastrodin had little effect while puerarin had certain (the IC was 17.56g L) on DPPH free radical-scavenging activity when the concentration of the two were 200g L and 50 / / 5g/L, respectively. The solubility of gastrodin in water