降解赤霉病菌毒素Tri101基因的原核表达及抗体制备.pdfVIP

植物病理学报 ACTAPHYTOPATHOLoGICASlNlCA 40(5)：469-474(2010) 降解赤霉病菌毒素Tril01基因的原核表达及抗体制备 闫海霞1”，杨丽荣2，薛保国孙，段立清1，孙虎2，全鑫2 (1内蒙古农业大学林学院，呼和浩特010019；2河南省农业科学院植物保护研究所。郑州450002) Western 75．45 256 000。Western blot分析表明制备的抗体与原核细胞体外表达的Tril01蛋白可以特异性结合，表明该抗体的特异性良 物学功能、细胞定佗以及在其它植物中的表达等奠定了基础。 blot 关键词：TrilOl基因；原核表达；多克隆抗体；Western and antiserum of reductionTril01 Prokaryoticexpression preparation toxicity gene from砌sarium SchwabeYAN graminearum Hai—xial．-，YANG Li—ron92，XUEBao．gu02， DUAN Xin2 of Li—qing‘，SUNHu2，QUAN(1 Forestry，Inner College MongoliaAgriculturalUniversity，Hub．hot Protection of 010019，China；2PlantInstitute，HenanAcademyAgricultural 450002，China) Science，Zhengzhou Tril01 ofFusarium Schw．was Abstract：The bvRT．PCRand tothe gene graminearumamplified ligated recombinant wasthentransformedinto叵coli expressionvector．pGEX4Te．Theplasmid．pGEX-4T2／Tril01 BL21strainandinducedIVrG．TheresultsofSDS—PAGEandWesternblot showedthattheTrilOl by analysis was molecularoftherecombinantfusion is75．45kDa．consistent gene highlyexpressed．Theweight protein withthe rabbit anti—GST-Tril01 was predictedresult．Polyclonal successfully antibody preparedbyusingpurl- fledTril01 as ELISAtiterofantiserumGST．Tril01iS than proteinimmun