白念珠菌消化道感染对脾虚小鼠肠组织IgA变化的影响韩晓伟.pdfVIP

第11卷 第 7 期 辽 宁 中 医 药 大 学 学 报 Vol. 11 No. 7 2009 年 7 月 JOURNAL OF LIAONING UNIVERSITY OF TCM Jul . ，2009 白念珠菌消化道感染对脾虚小鼠肠组织IgA变化的影响 韩晓伟，陈文娜，马贤德，陈殿学，雷 萍，关洪全 110032 摘 要：目的：探讨机体在脾虚状态下对消化道感染白念珠菌的易感性。方法：本实验采用饮食失节加劳倦过 度的复合因素制备小鼠脾虚证模型，经消化道感染白念珠菌，观察小鼠的体重变化及一般状况的改变，并检测粪便 中的活白念珠菌数，应用酶联免疫吸附法（ELISA）测定脾虚模型小鼠肠组织表达的免疫球蛋白A (IgA)的含量。结 果：脾虚感染组小鼠的体重下降明显（ P0.01），且一般状况欠佳，便活菌数持续增高，小肠组织IgA含量明显低于其 它各组（P0.01）。结论：机体在脾虚状态下对消化道感染白念珠菌的易感性增强。 关键词：白念珠菌；脾虚证；感染；IgA ( ) 中图分类号：R285.5 文献标识码：A 文章编号：1673-842X 2009 07- 0205- 03 Effect of Alimentary Tract Infection of Candida Albicans on IgA of Small Intestine Tissue in Mice with Spleen Deficiency Syndrome HAN Xiao-wei,CHEN Wen-na,MA Xian-de,CHEN Dian-xue,LEI Ping,GUAN Hong-quan （Liaoning University of Traditional Chinese Medicine, Shenyang 110032,Liaoning,China） Objective: To investigate the susceptibility of alimentary tract infection of Candida Albicans Abstract： in mice with spleen deficiency syndrome. Method: The animal model of spleen deficiency was prepared by using of irregular-feeding and overstrain methods，and the mice were infected by Candida Albicans through alimentary tract. Mice in each group were observed changes in body weight and general condition;The number of living Candida albicans in fecal was detected; The expression of immunoglobulin A (IgA)content in intestinal tissue of spleen deficiency mice were detected with application of enzyme-linked immunosorbent assay (ELISA). Results: The weight of spleen deficiency infected mice decreased significantly (P0.01),with the addition of poor general condition;The number of fecal living bacteria