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ECS Transactions, 33 (7) 135-142 (2010)
10.1149/1.3484770 © The Electrochemical Society
Fluorescence Activity of Green Fluorescent Protein in Ionic Liquids
L. M. Haverhalsa a a a
, C. L. DaBronzo , J. L. Schlessman , W. M. Reichert ,
b a
H. C. De Long , and P. C. Trulove *
a Department of Chemistry, U.S. Naval Academy, Annapolis, MD 21402, USA
b Air Force Office of Scientific Research, 875 North Randolph Street, Arlington, VA
22203, USA
In this study the fluorescence activity of a mutant form of Green
Fluorescent Protein (GFP), GFPuv, was explored in ionic liquid
solvents. Purified GFPuv was obtained and experiments probing
the fluorescence spectral properties of GFPuv in a series of
solvents were undertaken. Data show that GFPuv is fluorescent in
several ionic liquid solvents when sufficient water is also present,
and the amount of water (in the solvent system) is critical in
determining the fluorescent activity. Results are interpreted with
regards to previous Hofmeister series correlations in attempt to
rationalize solvating and stabilizing properties of the solvent
systems investigated.
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