扇贝裙边糖胺聚糖对6-OHDA诱导SH-SY57细胞氧化应激与凋亡的保护机制及研究.pdf

螂㈣ onthe mechanismof Study protective scallop on6-OHDA-inducedin SH-SY5Ycells apoptosis Abstract theeffectof the Objective：Toinvestigate scallop contentof of and MDA，theactivityLDH，the the ofseveral apoptosis expression and inSH··SY5Ycellsinduced6-OHDAandthen apoptosis-mlatedgenesproteins by the mechanismofSS-GAGon neurons． studyprotective dopaminergie Methods：HumanneuroblastomaSH—SY5Y divided cellswere intocontrol group， 6-OHDA and3treatmenttreatedwitll6-OHDAandSS-GAG destroy group groups and800 contentofMDAandthe ofLDHwere (200，400 Ixg枷)．The activity measured， themRNA ofBcl-2andBax wereevaluatedreal-timethe expressions genes by PCIL contentof Cin were Western the Cyt by protein cytoplasmanalyzed blotting，and of andtheactivationof Wasmeasuredflow percentageapoptosis Caspase-9 by cytometry． thecontrol itemsofthe6-OHDA have Results：Compared州虹lgroup，all group destroy remarkabledifferences contentofMDAandthe ofLDHwere PO．01)：the activity increased，theof