中药活血方对脊髓型颈椎病微循环影响实验研究.pdf

目 录 中文摘要···················································1 英文摘要 ···················································3 引 言 ····················································5 正 文 ·····················································7 1 实验材料和方法 ··········································7 1.1实验对象··················································7 1.2主要实验仪器及试剂 ········································7 1.3 试验方法 ·················································7 2.实验结果···············································13 3.讨 论 ················································15 4.存在的问题和展望 ······································35 结 论 ··················································37 参考文献 ··················································38 附 录 ··················································45 综 述 ···················································48 致 谢 ····················································54 攻读学位期间发表的学术论文目录 ······················55 作者声明 ·················································56 个人简历 ·················································57 中 文 摘 要 目 的：通过动物实验 探讨 脊髓型颈椎病 (cervical spondylotic Myclopathy，简称CSM)脊髓受压后兔后肢活动功能变化 情况，以及脊髓受压后血管内皮生长因子（VEGF ）、血栓素 A (TXA )、 2 2 前列腺素 I (PGI )的变化，并观察中药对改善兔后肢活动功能的作 2 2 用及对VEGF、TXA 、PGI 等指标的影响，了解脊髓型颈椎病微循 2 2 环改变及中药改善微循环情况，为中药在脊髓型颈椎病保守 治疗中的使用提供指导。 方 法：在颈椎椎体前臵入螺丝钉建立兔颈脊髓腹侧慢性受压动物 模型，造模对照组 8 只，造模后无特别处理；造模给药组 8 只，造模后 给中药活血方；空白对照组8 只，手术入路同造模组,不臵入螺钉假手术 处理。造模成功后进行神经功能评定。6 周后处死动物。取得标本后用 EnVision 两步法检测VEGF；用放射免疫法检测TXA 、PGI 。化染色；保 2 2 结 果：颈脊髓慢性持续受压后兔后肢活动功能受限，予活血化瘀 中药后兔后肢活动功能改善，评分明显增高。空白对照组 VEGF 表达少, 造模对照组脊髓受压迫后表达增加,造模给药组较造模对照组表达明显 增加。空白对照组PGI /TXA 处于正常状态，脊髓受压后脊髓组织中PGI 2 2