拟南芥MAPKKX参与ABA调控主根生长分子机制初探.pdfVIP

摘要 为了适应复杂多变的外部环境，植物形成了多种多样的信号转导途径，从而使细胞 能够做出适当的反应。在这些机制中，其中丝裂原活化蛋白激酶(MAPK)级联途径普 遍存在于真核生物中。丝裂原活化蛋白激酶途径由三种信号转导分子组成，丝裂原活化 ABA抑制主根生长仍不清楚。 方面与野生型没有差别，而在50 ABA处理的情况下，突变体的主根伸长是野生型的 t．tM MAP 实酵母双杂的结果，我们构建了双分子荧光互补的一系列载体。 以上的结果表明MAPKKKX参与了ABA调控的主根生长过程。 关键词：拟南芥，MAPKKKX，ABA，主根 ¨ ABSTRACT Plantshave various transductioninordertomodulatediversecellular developedsignal pathways for to environment these responsesadaptationchanging conditions．Among arefundamental conservedin proteinkinase(MAPK)cascadessignalpathways eukaryotes． kinasecascadesconsistofthree signal Mitogen-activatedprotein transducing MAPKKsandMAPKs．In to CanactivateMAPKK stimuli，MAPKKK response by inturn MAPK encodes activates Arabidops西∽rabidopsisthaliana)genome byphosphorylation．The 0MAPKKsand20MAPKs．ithasbeen thatMAPKcascadeshave 60一80 MAPKKKs．1 reported predicted an roleinABA isnotclearwhetherMAPKcascadesinvolvedin impoaant signaltransduction，however,it in the root modulatedABA primarygrowth by Arabidopsis． WeidentifiedT-DNAinsertion mutant ofMAPKKKin homozygousmapkkkx family thereforeuseditto thefunctionofMAPKKKXin root modulatedABAin growth analyze primary by A Was