小鼠桑椹胚和早期囊胚玻璃化冷冻保存技术的研究.pdf

OPS法玻璃化冷冻小鼠囊胚体外培养24h后扩张率达到71．15％，显著高于两步细管 法口如．05)。两步OPS法和两步细管法冷冻小鼠桑椹胚，囊胚化率分别为93．44％和 96．72％，两者间无显著差异。 平衡O．5 椹胚(42．86％，12．53绚口o．05)。 关键词：玻璃化冷冻，细管法，OPS法，桑椹胚和囊胚，小鼠 Ⅱ Abstract onvitrification ofmousemorula Study preservationtechnology and earlyblastocyst Cui ofClinical Cheng(DepartmentVeterinaryMedicine) DirectedProfessorLiu by Chang-song Inthis morulaand werevitrified StrawMethodandOPS study,mouseblastoeysts using for Method,afteffrozen w∽thawedDPBScontainedO．5MStlcro∞． 1-6d,embryosusing wet@culturedin for24h invitro rate Embryos TCMl99and andthe development pregnant andlitterrateaftertransferredwereobservedtoevaluatethebestmethodand for stage mollsemorulasand resultsindicatedasbelow： vitrifying blastoeysts．The 1、When werebalancedinEFS40for0．5minutestheOne embryos using Step hoursafter culturedinTCMl99for24 vilrificationand to Method,then thaw咄up to while hatched。When 70．59％morul蠲developedblastoeystsonly41．94％blastocysts were balancedinEFS40for1．0 ratesofmorulasand embryos minutes,the development aflcrthawedwere were blastocysts