《Profiling the repertoire of T-cell receptor beta-chain variable genes in peripheral blood lymphocytes from subjects who have recovered from acute hepatitis B virus infection.》.pdf

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《Profiling the repertoire of T-cell receptor beta-chain variable genes in peripheral blood lymphocytes from subjects who have recovered from acute hepatitis B virus infection.》.pdf

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CellularMolecularImmunology2(014】11．332-342 @2014CSlandUSTC．Allrightsreserved1672-7681／14$32．O0 www．nature．comlcm RESEARCHARTICLE ProfilingtherepertoireofT-cellreceptorbeta-c·hain variablegenesinperipheralbloodlymphocytesfr0m subjectswhohaverecoveredfromacutehepatitisBvirus infection JiezuanYang，_，JiajiaChen ，JianqinHe ，YiruiXie_，YixingZhu ，HongcuiCao2 andLanjnanLi’2 TheprofileofT．cellreceptorbeta．chainvariable(TRBV)genesusuallyskewsinsubjectswithvirusinfectionorcancer。 Thegenemeltingspectralpattern(GMSP)canbeusedtodeterminetheprofileoftheTRBVgenefamily．Toexplorethe portraitoftheTRBVfamilyinperipheralbloodlymphocytesfromsubjectswhohaverecoveredfromacutehepatitisBvirus infection(AHI)．peripheralbloodmononuclearcells(PBMCs)wereseparatedandfurthersortedintoCD4 andCD8 T．celIsubsets．ThemolecularfeaturesoftheTRBVcomplementarydeterminingregion3(CDR3)motifsweredetermined usingGMSPanalysis。WhenaGMSPprofileshowedasinglepeak，themonoclonallyexpandedTRBVgenewasclonedand sequenced．SkewedexpansionsofmultipleTRBVgeneswereobservedamongtheCD4 andCD8 T—cellsubsetsandthe PBMCs．ThefrequencyofmonoclonallyexpandedTRBVgenesintheCD8 T-celIsubsetwassignificantlyhigherthan thatoftheCD4+T．cellsubsetandthePBMCs．ComparedtoothermembersoftheTRBVgenefamily，TRBV11，BV15and BV20werepredominantlyexpressedintherepertoireofperipheraIbloodIymphocytesinrecoveredAHIsubjects．The relativelyconservedaminoacidmotifsofTRBV5．1andBV20CDR3werealsodetectedintheCD4 andCD8 T·celI subsets．TheseresultsdemonstratethepresenceofmultiplebiasedTRBVfamiliesinrecoveredAHlsubjects．TRBV11． BV15andBV20。especiallyfromtheCD8 T-cellsubset。mayberelevanttothepathogenesisofsubjectswithAHl_The preferentiallyselectedTRBV5．1andBV20withtherelativelyconservedCDR3motifmaybepotentialtargetsfor personalizedtreatmentsofchronicHBVinfection． CellularMolecu／a，／mmunology(2014)11。332—342；doi：10．1038／cmi．2014．22；publishedonIiBe2lApriI2014