《Proteomic Analysis of Fruit Bending in Cucumber （Cucumis safivus L.）.》.pdf

下载文档 降价啦

10
0
约 12页
2015-12-02 发布于河南
举报
版权申诉
保障服务

《Proteomic Analysis of Fruit Bending in Cucumber （Cucumis safivus L.）.》.pdf

1、本文档共12页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。

Availableontineatwww ProteomicAnalysisofFruitBendinginCucumber(CucumissafivusL．) WANGLi．1i，ZHANGPeng ，QINZhi—weiandZHOUXiu—yan CollegeofHorticulture，NortheastAgriculturalUniversity,Harbin150030,P．R．China InstituteofVegetable，ZhejiangAcademyofAgricuhureSciences,Hangzhou310021，P．R．China Abstract Incucumber，fruitshapeisan importantqualitycriterion，andfruitbendingisknowntolimitgrowth，yield，andtaste．To investigatethepost—transcriptionalchangesthatregulatefuritbendingandtobetterunderstandtheunderlyingmolecular mechanisms，wegeneratedaproteomicprofileoftheabdomenandbackofcucumberbendingfruit．Two-dimensionalgel electrophoresis(2一DE)allowedthedetectionofapproximately900distinctproteinspotsineachgel，32ofwhichwere differentiallyexpressedintheabdomenandbackofbendingcucmu berfruit．Tenofthedifferentiallyexpressedproteinswere analyzedusingmatrix-assistedlaserionizationtimeofflightmassspectrometry(MALDI-TOF／MS)．A searchofprimary databasesshowedthattheidentifiedproteinsareinvolved invariousmetabolicprocessesandcellulra responses，including photosynthesismetabolism，energymetabolism，defenseandstressresponse，nadregulation．Theidentifiedproteinsincluded largesubunitsofribulose-1，5一bisphosphatecarboxylase／oxygenase，whichraeinvolvedinphotosynhtesisandphotorespiratory metabolism，andisocitratedehydrogenase，whichisinvolvedinthetricarboxylicacidcycle．Itispossiblethatimbalances incatabolicandanabolicprocessesdirectlyaffectthebendingofcucmu berfruit．Thepredictedfunctionofthecobalamin— independentmethioninesynthaseisozymeiscloselyrelatedtoethylenebiosynthesis；fur itbendingmayberegulatedby ethylene，orbyethylenesignalingcrosstalkduringfruitdevelopment．The14—3—3proteinisusuallyconsideredtobea regulation-relatedprotein，whichplaysaroleinregulatingcellhyperplasia，celldifferentiationduringrgowth，andapoptosis duringsenescence．Involvementofguanosinetriphosphate(GTP)-bindingproteinsinsignaltransmissionisknowntoregulate thedevelopmentofcellsincucmu berfruitsandtoplayaroleinfruitshapeva