《Screen and effect analysis of immunostimulants for sea cucumber, Apostichopus japonicus》.pdfVIP

of ChineseJournal and OceanologyLirrmology 、，01．27No．1．P80—84，2009 Dol：lO．1∞7／s00343·009-0080-5 Screenandeffect ofimmunostimulantsforsea analysis cucumber,Apostichopusjaponicus木 LI Fengrong(郑风荣)卅， Jiye(李继业)”’，s盟?Xiuqin(孙修勤)卅”，ZHENG HAo Linhua(郝林华)”’ ’CollegeofMarineLifeScience，OceanUniversityofChina，Qingdao266003，China 什NorthChinaSeaEnvironmentalCenter,StateOceanic Monitoring 266033．China Administration，Qingdao 卅TheFirstInstitute ofOceanography,SOA，Qingdao266061，China ReceivedJuneI8，2008；revisionOct．8，2008 accepted lmmunostimulants resistance Abstract disease of animals mayimprove aquaculturebypromoting the ofthe of nonspecificimmunityresponseorganisms．Fivetypessaccharides，includingchitosan，yeast andlentinusedodes polysaccharide，burdockoligosaccharide，seaweedpolysaccharide polysaccharide。 were for useas screened immunostimulants saccharideswere potential byusingspectrophotometry．The into seacucumber,andthe and injectedApostichopusjaponicus，a lysozymesuperoxidedismutase(SOD) activitiesoftllecoelomicfluid were and slime monitoredinsixconsecutiveresults epidermal days．The showt}Iatthe of theanimal’scoelomicfluidwas stimulatedon lysozymeactivity significantly day2．day 4and 6afterthe ofthesaccharides effects ofchitosanand day injection