《六倍体普通小麦中国春PBF编码基因的克隆及序列分析 Cloning and Sequence Analysis of PBF Encoding Genes from Hexaploid Common Wheat Chinese Spring (Triticum aestivum L.)》.pdfVIP

ofAnhui 责任编辑朱琼琼责任校对卢瑶 安徽农业科学，Journal A西．Sci．2013，41(11)：4740—4744 六倍体普通小麦中国春PBF编码基因的克隆及序列分析 郭丽娜，高翔+ (西北农林科技大学农学院，陕西杨凌712100) 摘要[目的]探讨作为模式小麦中国春PBF转录因子编码基因的多样性。[方法]以中国春小麦品种为材料，利用基因特异引物进行 PCR扩增，经过胶回收，将目标片段连接至克隆载体上，随机选择阳性质粒测序。序列拼接与比对、信号肽预测、二级结构预测、亚细胞 国春基因组中克隆得到25条编码序列，根据序列相似性，将其聚成3种等位类型，亚基2个位点存在SNP，且都导致编码氨基酸残基的 改变，并影响到最终编码产物的二级结构。[结论]PBF亚基编码序列在中国春小麦中极端保守，但仍然存在变异。该结果为评价小麦 籽粒储藏蛋白亚基的表达效率提供了理论参考。 关键词普通小麦；PBF转录因子；克隆；序列分析 中图分类号$512．1文献标识码A 文章编号0517—6611(2013)11—04740—05 and ofPBF Genesfrom CommonWheatChinese aes疗vum CloningSequence．衄aysisEncoding Hexaploid Spring(TriticumL．) GUOLi·naetal of AF 712100) (CoUegeAgronomy，NorthwestUniversity，Yangling，Shaanxi aimedto the ofPBF fromcommonwheatChinese Abstract[Objective]Thisstudy investigatepolymorphismencodinggenes aestwum colnnlonwheatChineseasthe were and to L．)．[Method]Using Spring experimentalmaterial，gene—specificprimersdesignedapplied the DNAofChinese were and intothe vector．Positivecloneswererandom— amplifygenomie Spring．PCRproductsisolated，purifiedligated cloning selectedfor seriesofseftwares andMEGAwere for as- ly sequencing．A includingDNAMAN，Signalp，PSIPRED，Nuc—PLoeemployedsequence and and structure wellas ofsubcellularlocationand alignment，signal secondary sembly peptideprediction，primary prediction，asanalyses phylo— betweenthePBF membersGra from in wereobtainedthe of geneticrelationships family mineae．[Result]Twenty—fivetargetsequences genome commonwheatChinese wereclassifiedintothreecl