《PDCD5在系统性红斑狼疮患者外周血单个核细胞中表达的初步研究》.pdfVIP

PDCD5在系统性红斑狼疮患者外周血 单个核细胞中表达的初步研究 赵丽娜，赵杰。贺宝玲，张秀军’ (华北煤炭医学院，河北唐山063000) 情况，探讨其可能作用机制。方法应用免疫细胞化学和蛋白免疫印迹法检测45例SLE活动期患者、22例稳定期患 者和50例健康对照者PBMC中PDCD5蛋白的细胞定位和表达情况。结果SLE活动期患者PDCD5蛋白的阳性表 达率高于正常对照组(PO．01)；活动期患者PDCD5蛋白的表达水平高于稳定期患者和正常对照，稳定期患者高于 体滴度具有相关性。结论PDCD5可能作为淋巴细胞凋亡的正性调控基因，在SLE发生和发展中具有重要意义。 关键词：系统性红斑狼疮；程序性死亡因子5；凋亡；外周血单个核细胞 中图分类号：115923．24文献标志码：A 文章编号：1002-266X(2010)20-0022-03 of celldeath5in bloodmononuclearcells Expressionprogrammed peripheral from with patientssystemiclupuserythematosus Z^fA0厶一舭，Z而『A0Jie，HE Xiu Bao—ling，ZHANGjun ChinaCoalMedical (North College，Tangshan063000，P．R．China) To the of celldeath bloodmononuclear 5(PDCD5)in Abstract：Objectivestudyexpressionprogrammed peripheral with to theroleofPDCD5intheonsetof cells(PBMC)fromp=ientssystemiclupuserythematosus(SEE)，andinvestigate localizationand ofPDCD5inPBMCfrom45activeSLE inactiveSLE SLE．MethodsThe expression patients，22 patients 50 controls．ResultsPDCD5 WaS inPBMC．The ofPDCD5Wesmain- andhealthy proteinexpressedwidely positivestaining foundin rateofPDCD5fromactiveSLE was thanthatinnormaloon· ly cytoplasm．Thepositive patientssignificantlyhigher levelofPDCD5inactiveSLE W88 thanthatofinactiveSLE trois(P0．01)．Theexpression patientssignificantlyhigher pa- thatofnormalcontrols．PDCD5levelofinactiveSLE WSg thanthatofnormal tientsand expression patientssignificantlyhigher correlationwasfoundbetweenthePDCD5 in fromactiveSLE and controls(P0．01)．Significant expressionPBMC patients clinical