注射和浸泡免疫疫苗后牙鲆免疫相关基因表达的变化的研究.pdf

和鳃中表达高峰出现在72h，在脾中表达高峰出现在7d，IgM 在鳃中表达高峰出 现在96h，在脾和头肾中表达量在14d 内逐渐上升，各基因表达最高值是对照组 的2-20 倍。结果表明，注射组各组织几乎所有基因的转录水平均高于浸泡组， 且脾脏、头肾中最高值出现时间早于浸泡组，但鳃中各基因峰值出现时间与浸泡 组相同或晚于浸泡组。研究结果丰富了免疫相关基因的分子研究，为疫苗的免疫 途径及免疫效果评价提供了数据。 关键词： 牙鲆；鳗弧菌疫苗；注射免疫；浸泡免疫；免疫相关基因 万方数据 Variations of immune related genes expression in flounder (Paralichthys olivaceus) injected and immersed with vaccine Abstract Vaccination is an effective method for controlling diseases of fish. At present, researchers have paid more attention to variations of immune related genes expression to evaluate the immune effect of vaccine, besides the changes of antibody level of serum and mucus, enzyme activity, number of lymphocytes, respiratory burst, complement activity and so on. The toll-like receptor(TLR) Signal pathway members, TLR2, TLR5M, myeloid differentiation factor 88(MyD88) and nuclear transcription factor(NF)- κB, cytokines such as interleukin-1β (IL-1β), IL-6, Interferon(IFN) γ, tumor necrosis factor α (TNF α) and chemokines , Humoral factor, c-type lysozyme and heat shock protein(HSP) 70 are the key factors in inducing inflammatory and immune cell migration, immune regulation and stimulating the proliferation of T cells and B cells. Major histocompatibility complex class I (MHC Ⅰ), major histocompatibility complex class II (MHC II), T cell surface receptor CD4, T cell surface receptor CD8 and immunoglobulin M (IgM) can play key roles in different stages such as presentation and recognition of antigens an