集核酸抽提、核酸扩增及在线检测为一体的微流控芯片的研制.pdf

摘要 Abstract most areasinthefield Microfluidic isoneoftheimportant microanalyticalsystems chip．based ultimate istO ofmicrototal Lab…ona ofwhich analysissystems(gTAS)orchip，the objective allmain integrateanalyticalfunctions，includingsampleintroduction，sample anddetection a ofthe fieldin important separation operations，onsinglechip．Atpresent，one microfluidic—based is on aimsat a microanalyticalsystems analysischip，whichachieving genetic microfluidic with sample··in·-answer-out fullyintegrated geneticanalysissystem capability．The fornucleicacid workisfocusedon an microdevice purification， present developingintegratedglass detection． online amplification，and In microfluidicnucleicacidsextractionandits with I，the chip·based integration Chapter unitsarereviewed．Variousmethodsfornucleicacidextractionon subsequentgeneticanalysis chip， ofDNAon and ofdifferentfunctional PCRandisothermal intergration units，are amplificationchip described． comprehensively In methodfornucleicacidextraction SPE described． 2，a