Molecular Markers Associated with Starch Content and Implications for Sugarcane Introgression Breeding Using Saccharum spontaneum.pdfVIP

JournalofAgriculturalScienceandTechnologyB2(2012)1127-1137 Earliertitle：JournalofAgriculturalSciencena dTechnology，ISSN 1939-1250 MolecularMarkersAssociatedwithStarchContentand I●m Pl■l■cat-ionst-or5●IugarcaneI●ntrogressI■onB一reed-i■n!：I UsingSaccharum spontaneum MarvellousM abezaZhou一，AndruSuman2andCollinsKimbeng ． SouthAfricanSugarcaneResearchInstitute，MtEdgecombe，Durban,KwaZulu-Natal 2．SchoolofPlant,EnvironmentalandSoilSciences，LouisianaStateUniversity,BatonRouge，Louisiana，USA 3．SugarResearchStation，LSUAgCenter,StGabriel,Louisiana，USA Received：July14，2012／Published：November20，2012 Abstraet：IntrogressionwithSaecharum spontaneum intendedtobroadenthegeneticbaseofsugarcaneresultedinanincreasein desirablegenesforbroaderadaptabilityaswellasundesirablegenesforhighstarchcontent ． Markerscouldprovideaquickand efficientmethodofscreeningparentalgenotypesofrlowstarch．Theobjectivesofthisstudyweretoidentifypotentialmarkers associated with starchin spontaneum population na devaluatetheirpotentialofrscreeningofrstarch content ． Dataofrstrach contentand(simplesequencerepeats)SSRmarkerswerecollectedfrom51 spontaneumclonesgrowninreplicatedpots．The mixedprocedureofstatisticalanalysissystem (SAS)wasusedtodeterminemarkerssignificantlyassociatedwithstrach．Thirty．nine outof357polymorphicmarkersweresignificnatly(尸0．05)associatedwithstarchcontent．Eighteenwerepositivelyassociatedand 21werenegativelyassociated．Thepresenceofapositivemarkerproduced39％ morestarchthna absencewhiletheabsenceofa negativemarkerproduced 57％ morestrachthna presence．Selectingpraentsusingnegativemrakersmaybemoree衔cientthna selectingusingpositivemarkers． Keywords：Moleculra markers，strachcontent，introgression，Saccharum spontaneum 1．Introduction c