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Abstract
Sensitizedfluorescenceof ferulic its
chlorogenicacid(CA)and
acid(FA)and
in ofChineseherbalmedicinewerestudied.Fluorometricmethodanda
applicationanalysis
sensitizedfluorescencemethodfor determinationofCAin
thin-layer scanning quantitative
Chineseherbal asFlosLonicerae in
medicine,such Japonicae(FLJ,JinyinhuaChinese)etc,
new
wereestablished.Amethodfor ofFAinsomemedicinalwas
qualitativeanalysis crops
proposed.
Threewereincludedinthisthesis:
parts
1.The ofCAwasstudied.The Can
hydrolyzationprocess hydrolysisproductproduce
fluorescencewhichisthesameasthatofcaffeic of
acid.Additionboraxand
cetyltrimethyl
ammonium CAsolutionCan sensitized
bromide(CTAB)into
produce
suitableconditionforthe ofsensitizedfluorescencewas the
production investigated,and
was
method to ofChineseherbalmedicinewhichcontainsCA.
appliedanalysis
2.Anew sensitizedfluorescencemethodwas forthe
thin-layer scanning proposed
ofCAinFLJ.Thismethodwasdone the of via
analysis by separate FLJ
firstly components
boraxandCTABsolution
thin—layerchromatography,andsecondly on
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