猪囊尾蚴半胱氨蛋白酶tscl-1基因的表达.pdfVIP

摘要 囊虫瘸是鑫猪带绦受懿幼虫猪囊建蚴感染猪或入露零i趣翁入鸯共患病，半骁懿酸蛋囱酶 (Cysteineprotease)广泛地存在于人类、寄生虫等多种动物体内。由予寄生虫半胱氨酸蛋囱酶在宿主 寄囊虫耩嚣关系上起着重簧捧耀，并且可律为寄生虫病纯攀治疗帮免疫预防的一个极好豹作用靶点， 使人们越来越关注该酶戆研究。本实验黠猪囊尾蚴戆半熬氮酸蛋自酶蒸霹进行了竟隆，袭达，为该 酶的进一步研究奠定了基础。 ’ I期Not 量大小’为61Ku发右，能被猪囊尾蚴阳性血清所识另《；海了获得活性更好的蛋白，构建了真孩表达 子，采髑(}418浓度撵度避符筛选，获德了高拷廷重组菌撩，PCR检测表咀有秘兹基粼魏整合；悉 Tscl．1蛋自基因在毕赤酵母中成功表达，表达产物的分子凝为42薹沁左右，能被猪囊尾蚴蹋性血清所 谈裂。 关键词：猪囊尾蚴，TsCL：1基因，表达，免疫艨性’ Abstract causedTaeniasoliumisan zoonoticdisease．The exist Cysticercosisby important Cysteineproteases inhumanand of variousessentialrolesin extensively parasites．Cysteineproteasesparasitesplay interactionsandhave attentionas for or host·parasite gainedsignificant targetschemotherapy this Weclonedand TsCL-1fromTaeniasolium immunoprophylaxis．InstudM expressedgene metacestode， forthefurtherstudieson preparing cysteinepmteases． TotalRNAwasextractedfromTaeniasoIium TsCL-I were metacestode，Thegonespecificprimers DNAStarsoRware．A960 was RT-PCR．After designedusing bpspecificfragmentamplifiedby digested withEcoRI andNot wasinsertedintothe vector I，the pmkaryotic fragmentsubsequently expression andtransformedintoE．coliBL21and levelof ofthe pGEX-4T-1 sequenced．Ahi．gh expression recombinantwasinducedIPTGThe were SDSand protein by expressedproductsanalyzedby of that