ESR基因在苏姜猪世代选育中的遗传变异及与猪群繁殖性状的关联.pdfVIP

中国农业科学 2012,45(4):768-773 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2012.04.018 ESR 基因在苏姜猪世代选育中的遗传变异及 与猪群繁殖性状的关联 王宵燕, 何庆玲, 经荣斌, 宋成义 （扬州大学动物科学与技术学院，江苏扬州 225009 ） 摘要：【目的】探讨苏姜猪ESR 基因的Pvu Ⅱ酶切位点多态性在世代选育过程中的遗传变异及与猪群繁殖性状 的关系。【方法】采用PCR-RFLP 的方法对906 头、2—6 世代苏姜猪ESR 基因Pvu Ⅱ酶切位点的多态性进行分析， 并采用最小二乘法和单因子方差分析该位点多态性与苏姜猪群的繁殖性状的关联效应。【结果】ESR 基因在苏姜猪 的第 2—6 世代中B 等位基因频率呈现出不断增加的趋势，BB 基因型个体从第三世代开始出现，并且频率呈现出 上升的趋势。仅考虑公猪基因型时，与配母猪总产仔数、产活仔数及死胎数之间无显著差异，但不同基因型公、 母猪交配后母猪产仔数则有显著差异 （ P＜0.05）。【结论】ESR 基因的Pvu Ⅱ酶切位点的多态性对不同基因型公、 母猪交配后母猪的产仔数有显著影响。 关键词：ESR 基因; 苏姜猪; 遗传变异; 繁殖性能 Genetic Variation of ESR Gene in Different Generations of Sujiang Pigs and the Impact on Reproductive Traits of Pigs WANG Xiao-yan, HE Qing-ling, JING Rong-bin, SONG Cheng-yi (College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu) Abstract: 【Objective 】This study was aimed to analyze Pvu Ⅱpolymorphism of ESR gene in different generations of Sujiang pigs and the correlation between the polymorphism and the reproductive traits of Sujiang pigs. 【Method 】 PCR-RFLP was used to diagnose polymorphism of ESR gene in 906 Sujiang pigs of 2-6 generations. Least square method and one-way anova were performed to evaluate the association of this polymorphism site with reproductive traits of pigs. 【Result 】The frequency of allele B of ESR gene in Sujiang pigs of 2-6 generations showed an increasing trend. Pigs with BB genotypes appeared in the third generations and then presented an upward trend in the frequency. There was no significant relationship between the polymorphism of ESR gene and litter size of sows mated to different genotypes of boars. But they had