编码棉铃虫化学感受蛋白cDNA的克隆及序列分析.pdfVIP

中国农业科学 2005,38(11):2233-2239 Scientia Agricultura Sinica 编码棉铃虫化学感受蛋白cDNA的克隆及序列分析 王桂荣，吴孔明，郭予元 ( 中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室，北京 100094) 摘要：从棉铃虫触角中克隆了一条全长722bp的cDNA序列，命名为CSPHarm。CSPHarm 阅读框全长381bp， 编码127个氨基酸残基，推导的氨基酸序列具有昆虫化学感受蛋白的典型特征。CSPHarm与已报道的其它昆虫的 化学感受蛋白的氨基酸序列有很高的同源性，因此，CSPHarm是一个编码棉铃虫化学感受蛋白的基因。CSPHarm具 有强烈的亲水性，但是在第20～30位的氨基酸组成一个亲脂性的结构，这可能是结合亲脂性气味物质的区域。半 定量RT-PCR研究结果显示，CSPHarm在棉铃虫头、胸、腹、足、翅和触角等组织中表达量都很高，在不同组织中 的表达量没有明显的区别，在卵、幼虫、蛹和成虫体内也都有表达，在卵中表达量相对较低，在成虫体内表达量 较高。 关键词：棉铃虫；化学感受蛋白；基因克隆 Cloning and Sequence Analysis of a cDNA Encoding Chemosensory Protein in Helicoverpa armigera WANG Gui-rong, WU Kong-ming, GUO Yu-yuan (State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094) Abstract: A full-length cDNA, CSPHarm, was cloned from antennae of Helicoverpa armigera . The open reading-fragment of CSPHarm was 381bp, encoding 127 amino acid residues. This deduced amino acid sequence shared some common structural features with chemosensory proteins in insects. It also shared high identity with chemosensory proteins from other insects. Therefore, CSPHarm was a cDNA encoding chemosensory protein of H.armigera . CSPHarm displayed a strong hydrophilicity all along the molecular, except for the hydrophobic domain between residues 20-30, which was likely binding domain of hydrophobic compounds. Semi-quantitative RT-PCR analysis showed that CSPHarm was highly expressed in head, thorax, abdomen, leg, wing and antenna of H. armigera . No obrious difference at expressed level of CSPHarm among these tissues. CSPHarm was also expressed in egg, larva, pupae and adult and relatively low expressed in egg and highly expre